Spontaneous Ca2+ transients in rat pulmonary vein cardiomyocytes are increased in frequency and become more synchronous following electrical stimulation
| Autor: | Robert M. Drummond, Francis L. Burton, Edward G. Rowan, Alasdair Henry, Niall Macquaide, Andrew Rankin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
RM Sarcolemma Physiology Chemistry Ryanodine receptor Stimulation Cell Biology 030204 cardiovascular system & hematology Calcium in biology Pulmonary vein 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Biophysics Extracellular Fluorescence microscope Molecular Biology Intracellular |
| ISSN: | 0143-4160 |
| Popis: | The pulmonary veins have an external sleeve of cardiomyocytes that are a widely recognised source of ectopic electrical activity that can lead to atrial fibrillation. Although the mechanisms behind this activity are currently unknown, changes in intracellular calcium (Ca2+) signalling are purported to play a role. Therefore, the intracellular Ca2+ concentration was monitored in the pulmonary vein using fluo-4 and epifluorescence microscopy. Electrical field stimulation evoked a synchronous rise in Ca2+ in neighbouring cardiomyocytes; asynchronous spontaneous Ca2+ transients between electrical stimuli were also present. Immediately following termination of electrical field stimulation at 3 Hz or greater, the frequency of the spontaneous Ca2+ transients was increased from 0.45 ± 0.06 Hz under basal conditions to between 0.59 ± 0.05 and 0.65 ± 0.06 Hz (P |
| Databáze: | OpenAIRE |
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