ZBTB10 binds the telomeric variant repeat TTGGGG and interacts with TRF2
| Autor: | Yunyu Shi, Alina Bluhm, Suman Wang, Michal Levin, Nikenza Viceconte, Dennis Kappei, Grishma Rane, Fudong Li, Sandra Ritz, Falk Butter |
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| Rok vydání: | 2018 |
| Předmět: |
Telomere-Binding Proteins
Biology Genome Chromosomes Shelterin Complex 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Genetics Humans Telomeric Repeat Binding Protein 2 Transcription factor Molecular Biology 030304 developmental biology Repetitive Sequences Nucleic Acid Zinc finger 0303 health sciences Binding Sites Binding protein Telomere Homeostasis Telomere Cell biology Nucleoprotein DNA-Binding Proteins Repressor Proteins chemistry Rap1 030217 neurology & neurosurgery DNA Protein Binding |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 |
| Popis: | Telomeres are nucleoprotein structures at the ends of linear chromosomes and present an essential feature for genome integrity. Vertebrate telomeres usually consist of hexameric TTAGGG repeats, however, in cells that use the alternative lengthening of telomeres (ALT) mechanism, variant repeat sequences are interspersed throughout telomeres. Previously, it was shown that NR2C/F transcription factors bind to TCAGGG variant repeats and contribute to telomere maintenance in ALT cells. While specific binders to other variant repeat sequences have been lacking to date, we here identify ZBTB10 as the first TTGGGG-binding protein and demonstrate direct binding via the two zinc fingers with affinity in the nanomolar range. Concomitantly, ZBTB10 co-localizes with a subset of telomeres in ALT-positive U2OS cells and interacts with TRF2/RAP1 via the N-terminal region of TRF2. Our data establishes ZBTB10 as a novel variant repeat binding protein at ALT telomeres. |
| Databáze: | OpenAIRE |
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