Strategies for the release of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in an enzymatic hydrolyzate of α-lactalbumin
| Autor: | Richard J. FitzGerald, Solène Le Maux, Alice B. Nongonierma, Joël Hamayon |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chemical Phenomena Dipeptidyl Peptidase 4 Tandem mass spectrometry Dipeptidyl peptidase Inhibitory Concentration 50 03 medical and health sciences Hydrolysis Tandem Mass Spectrometry Animals IC50 Dipeptidyl peptidase-4 chemistry.chemical_classification Lactalbumin Dipeptidyl-Peptidase IV Inhibitors 030109 nutrition & dietetics Chromatography Elastase Temperature General Medicine Hydrogen-Ion Concentration Milk Proteins Enzyme chemistry Biochemistry Cattle Chromatography Liquid Food Science |
| Zdroj: | Food & Function. 7:3437-3443 |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/c6fo00239k |
| Popis: | Bovine α-lactalbumin (α-La) contains numerous dipeptidyl peptidase IV (DPP-IV) inhibitory peptide sequences within its primary structure. In silico analysis indicated that the targeted hydrolysis of α-La with elastase should release DPP-IV inhibitory peptides. An α-La isolate was hydrolysed with elastase under different conditions using an experimental design approach incorporating 3 factors (temperature, pH and enzyme to substrate ratio (E : S) ratio) at 2 levels. The hydrolyzate generated at pH 8.5, 50 °C, E : S 2.0% (w/w) (H9) displayed the highest mean DPP-IV inhibition value at 3.1 mg mL(-1) of 75.8 ± 3.7% and had a half maximal DPP-IV inhibitory concentration (IC50) value of 1.20 ± 0.12 mg mL(-1). Five α-La-derived DPP-IV inhibitory peptides (GY, GL, GI, NY and WL) predicted to be released in silico were identified by liquid-chromatography tandem mass spectrometry (LC-MS/MS) within H9 and its simulated gastrointestinal digestion (SGID) sample. This preliminary study demonstrated the benefit of using a targeted approach combined with an experimental design in the generation of dietary protein hydrolyzates with DPP-IV inhibitory properties. |
| Databáze: | OpenAIRE |
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