HPV caught in the tetraspanin web?

Autor: Jérôme Finke, Lisa Hitschler, Klaus Boller, Thorsten Lang, Luise Florin
Rok vydání: 2020
Předmět:
Zdroj: Medical microbiology and immunology, 209(4):447-459
Medical Microbiology and Immunology
ISSN: 1432-1831
0300-8584
Popis: Tetraspanins are master organizers of the cell membrane. Recent evidence suggests that tetraspanins themselves may become crowded by virus particles and that these crowds/aggregates co-internalize with the viral particles. Using microscopy, we studied human papillomavirus (HPV) type 16-dependent aggregates on the cell surface of tetraspanin overexpressing keratinocytes. We find that aggregates are (1) rich in at least two different tetraspanins, (2) three-dimensional architectures extending up to several micrometers into the cell, and (3) decorated intracellularly by filamentous actin. Moreover, in cells not overexpressing tetraspanins, we note that obscurin-like protein 1 (OBSL1), which is thought to be a cytoskeletal adaptor, associates with filamentous actin. We speculate that HPV contact with the cell membrane could trigger the formation of a large tetraspanin web. This web may couple the virus contact site to the intracellular endocytic actin machinery, possibly involving the cytoskeletal adaptor protein OBSL1. Functionally, such a tetraspanin web could serve as a virus entry platform, which is co-internalized with the virus particle. Electronic supplementary material The online version of this article (10.1007/s00430-020-00683-1) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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