Salient brain entities labelled in P2rx7-EGFP reporter mouse embryos include the septum, roof plate glial specializations and circumventricular ependymal organs
| Autor: | María Teresa Miras-Portugal, Arturo Azcorra, Manuel Desco, Margaret Martinez de la Torre, Antonio R. Artalejo, Luis Alcides Olivos-Oré, Luis Puelles, María Benito-León, Maria Victoria Gómez-Gaviro, Marina Arribas-Blázquez, Rosa Gómez-Villafuertes, Felipe Ortega |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Comunidad de Madrid, Universidad Complutense de Madrid (España), Banco Santander, Fundación Ramón Areces, European Regional Development Fund (ERDF/FEDER), Ministerio de Ciencia, Innovación y Universidades (España), Fundación ProCNIC, Instituto de Salud Carlos III - ISCIII, Universidad Complutense de Madrid, Agencia Estatal de Investigación (España), Fundación Pro CNIC, Instituto de Salud Carlos III, European Commission, Comunidad de Madrid (España), Complutense University of Madrid (España), Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) |
| Rok vydání: | 2021 |
| Předmět: |
Histology
Green Fluorescent Proteins Central nervous system Mice Transgenic Biology Neuroprotection Ventricular hypothalamic organ Green fluorescent protein 03 medical and health sciences 0302 clinical medicine Ependyma P2rx7-EGFP mouse medicine Animals Postarcuate organ Receptor Neuroinflammation Biología y Biomedicina 030304 developmental biology Neurons 0303 health sciences General Neuroscience Purinergic receptor Brain Purinergic system P2RX7 PArcO medicine.anatomical_structure Circumventricular Organs P2X7 receptor Original Article Receptors Purinergic P2X7 Anatomy Neuroglia Neuroscience 030217 neurology & neurosurgery Ionotropic effect Embryonic brain |
| Zdroj: | Repisalud Instituto de Salud Carlos III (ISCIII) IMDEA Networks Institute Digital Repository IMDEA Networks Institute instname Digital.CSIC. Repositorio Institucional del CSIC e-Archivo. Repositorio Institucional de la Universidad Carlos III de Madrid Brain Structure & Function |
| Popis: | The purinergic system is one of the oldest cell-to-cell communication mechanisms and exhibits relevant functions in the regulation of the central nervous system (CNS) development. Amongst the components of the purinergic system, the ionotropic P2X7 receptor (P2X7R) stands out as a potential regulator of brain pathology and physiology. Thus, P2X7R is known to regulate crucial aspects of neuronal cell biology, including axonal elongation, path-finding, synapse formation and neuroprotection. Moreover, P2X7R modulates neuroinflammation and is posed as a therapeutic target in inflammatory, oncogenic and degenerative disorders. However, the lack of reliable technical and pharmacological approaches to detect this receptor represents a major hurdle in its study. Here, we took advantage of the P2rx7-EGFP reporter mouse, which expresses enhanced green fluorescence protein (EGFP) immediately downstream of the P2rx7 proximal promoter, to conduct a detailed study of its distribution. We performed a comprehensive analysis of the pattern of P2X7R expression in the brain of E18.5 mouse embryos revealing interesting areas within the CNS. Particularly, strong labelling was found in the septum, as well as along the entire neural roof plate zone of the brain, except chorioidal roof areas, but including specialized circumventricular roof formations, such as the subfornical and subcommissural organs (SFO; SCO). Moreover, our results reveal what seems a novel circumventricular organ, named by us postarcuate organ (PArcO). Furthermore, this study sheds light on the ongoing debate regarding the specific presence of P2X7R in neurons and may be of interest for the elucidation of additional roles of P2X7R in the idiosyncratic histologic development of the CNS and related systemic functions. This work was supported by grants “Red de Excelencia Consolider-Ingenio Spanish Ion Channel Initiative” (BFU2015-70067REDC) from the Ministry of Economy and Competitiveness (BFU2014-53654-P), BRADE-Comunidad de Madrid (S2013/ICE-2958), UCM-Santander (PR26/16-18B-3; PR75/18) and Fundación Ramón Areces Grant program (PR2018/16–02). Maria Benito Leon is recipient of a contract from the “Fondo de Garantía Juvenil, Comunidad de Madrid” CAM PEJD-2016/BMD-2572. Felipe Ortega acknowledges support from the Ramon y Cajal Program of the Spanish Ministry of Economy and Competitiveness (MEC: RYC-2013–13290). This work has been also supported by Comunidad de Madrid, project “S2017/BMD-3867 (RENIM-CM)”, co-funded by European Structural and Investment Fund. The CNIC is supported by the Ministerio de Ciencia, Innovación y Universidades and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015–0505). MVG has been supported by Ministerio de Ciencia, Innovación y Universidades, ISCIII-FIS grants PI18/00462 co-financed by ERDF, European Union (FEDER) Funds from the European Commission, European Union, “A way of making Europe”. |
| Databáze: | OpenAIRE |
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