Ebola-GP DNA Prime rAd5-GP Boost: Influence of Prime Frequency and Prime/Boost Time Interval on the Immune Response in Non-human Primates
| Autor: | Yan Zhou, Kathryn E. Foulds, Clement Asiedu, Michael Bailey, Juan I. Moliva, Emily Thompson, Mario Roederer, Daphne A. Stanley, Mitzi M. Donaldson, Nancy J. Sullivan, Hadar Marcus |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
lcsh:Immunologic diseases. Allergy CD4-Positive T-Lymphocytes Time Factors T cell Immunology Immunization Secondary Heterologous Biology CD8-Positive T-Lymphocytes medicine.disease_cause Antibodies Viral complex mixtures Prime (order theory) CD4+ 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Immunogenicity Vaccine T-cell vaccine medicine Vaccines DNA Immunology and Allergy Animals 030212 general & internal medicine prime Ebola Vaccines Immunization Schedule Original Research Ebola virus Hemorrhagic Fever Ebola CD8+ Ebolavirus Macaca fascicularis 030104 developmental biology medicine.anatomical_structure chemistry Immunization Ebola Female non-human primates lcsh:RC581-607 boost Immunologic Memory DNA CD8 |
| Zdroj: | Frontiers in Immunology Frontiers in Immunology, Vol 12 (2021) |
| ISSN: | 1664-3224 |
| Popis: | Heterologous prime-boost immunization regimens are a common strategy for many vaccines. DNA prime rAd5-GP boost immunization has been demonstrated to protect non-human primates against a lethal challenge of Ebola virus, a pathogen that causes fatal hemorrhagic disease in humans. This protection correlates with antibody responses and is also associated with IFNγ+ TNFα+ double positive CD8+ T-cells. In this study, we compared single DNA vs. multiple DNA prime immunizations, and short vs. long time intervals between the DNA prime and the rAd5 boost to evaluate the impact of these different prime-boost strategies on vaccine-induced humoral and cellular responses in non-human primates. We demonstrated that DNA/rAd5 prime-boost strategies can be tailored to induce either CD4+ T-cell or CD8+ T-cell dominant responses while maintaining a high magnitude antibody response. Additionally, a single DNA prime immunization generated a stable memory response that could be boosted by rAd5 3 years later. These results suggest DNA/rAd5 prime-boost provides a flexible platform that can be fine-tuned to generate desirable T-cell memory responses. |
| Databáze: | OpenAIRE |
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