Evaluation of interactions between RAW264.7 macrophages and small molecules by capillary electrophoresis
| Autor: | Yin-Zhen Wang, Yuanjia Hu, Feng-Qin Wang, Qiao-Qiao Li, Zhining Xia, Jian-Bo Wan, Peng Li, Feng-Qing Yang, Qian Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Capillary action Clinical Biochemistry Iridoid Glucosides Buffers 01 natural sciences Biochemistry Analytical Chemistry Cell Line 03 medical and health sciences chemistry.chemical_compound Mice Capillary electrophoresis Electricity Glucosides Animals Iridoids Receptor Naringin Chromatography Macrophages 010401 analytical chemistry Electrophoresis Capillary Paeoniflorin Small molecule Binding constant Cell aggregation 0104 chemical sciences 030104 developmental biology chemistry Flavanones Monoterpenes |
| Zdroj: | Electrophoresis. 38(6) |
| ISSN: | 1522-2683 |
| Popis: | In this study, the affinity interactions between RAW 264.7 macrophages and three small molecules include naringin, oleuropein and paeoniflorin were evaluated by affinity capillary electrophoresis (ACE), partial filling affinity capillary electrophoresis (PFACE) and frontal analysis capillary electrophoresis (FACE), respectively. The result indicated that 1) ACE (varying concentrations of cell suspension were filled in the capillary as receptor) may not be suitable for the evaluation of interactions between cell and small molecules due to the high viscosity of cell suspension; 2) PFACE can qualitatively evaluate the interaction, but the difference in viscosity between RAW264.7 suspension and buffer effects on the liner relationship between filling length and injection time, which makes the calculation of binding constant difficult. Furthermore, based on the PFACE results, naringin showed stronger interaction with macrophages than the other two molecules; 3) Taking advantage of the aggregation phenomenon of cell induced by electric field, FACE was successfully used to determine the stoichiometry (n = 5×109) and binding constant (Kb = 1×104 L/mol) of the interaction between RAW264.7 and naringin. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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