Identification of HIV transmitting CD11c+ human epidermal dendritic cells
| Autor: | Andrew N. Harman, Eric Hunter, Chloe M Doyle, Najla Nasr, Hafsa Rana, Jake W. Rhodes, Jake Lim, Caroline Royle, James Fletcher, Naomi R. Truong, Mark P. Kohout, Paul U. Cameron, Laith Barnouti, Jacob D. Estes, Michael Wines, Andrew J. Brooks, Ellis Patrick, Peter A. Haertsch, Rachel A. Botting, J. Dinny Graham, Kirstie M. Bertram, Toby M. Plasto, Anthony L. Cunningham, Heeva Baharlou, Muzlifah Haniffa, Orion Tong, Grahame Ctercteko, Martijn P. Gosselink, Melissa J Churchill |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine T-Lymphocytes Sialic Acid Binding Ig-like Lectin 3 General Physics and Astronomy HIV Infections 02 engineering and technology lcsh:Science Cells Cultured Antigen Presentation education.field_of_study Multidisciplinary integumentary system virus diseases hemic and immune systems Mononuclear phagocyte system 021001 nanoscience & nanotechnology Healthy Volunteers 3. Good health Cell biology Mucosal immunology Female 0210 nano-technology Sexual transmission Receptors CCR5 Science Primary Cell Culture Population Antigen presentation CD11c chemical and pharmacologic phenomena Biology Article General Biochemistry Genetics and Molecular Biology Virus 03 medical and health sciences Humans education Epidermis (botany) Dendritic Cells General Chemistry Virus Internalization CD11c Antigen 030104 developmental biology Epidermal Cells Cell culture HIV-1 lcsh:Q Epidermis |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-15 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Langerhans cells (LC) are thought to be the only mononuclear phagocyte population in the epidermis where they detect pathogens. Here, we show that CD11c+ dendritic cells (DCs) are also present. These cells are transcriptionally similar to dermal cDC2 but are more efficient antigen-presenting cells. Compared to LCs, epidermal CD11c+ DCs are enriched in anogenital tissues where they preferentially interact with HIV, express the higher levels of HIV entry receptor CCR5, support the higher levels of HIV uptake and replication and are more efficient at transmitting the virus to CD4 T cells. Importantly, these findings are observed using both a lab-adapted and transmitted/founder strain of HIV. We also describe a CD33low cell population, which is transcriptionally similar to LCs but does not appear to function as antigen-presenting cells or acts as HIV target cells. Our findings reveal that epidermal DCs in anogenital tissues potentially play a key role in sexual transmission of HIV. Composition and function of immune populations at barrier surfaces is crucial for response to infection. Here, the authors identify a population of dendritic cells in human epidermis, abundant in anogenital epithelia and distinct from Langerhans cells by surface phenotype and by high capacity for HIV infection and transmission. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|