Effects of Tear Substitutes on Conjunctival Epithelium of Mice
| Autor: | Antonio Micali, Felicia Ferreri, Domenico Puzzolo, Pasquale Aragona, Grazia Paladino |
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| Rok vydání: | 2007 |
| Předmět: |
Pathology
medicine.medical_specialty Conjunctiva Coloring agents conjunctival epithelium Methylcellulose Sodium Chloride Biology Conjunctival Epithelium Epithelium Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Hypromellose Derivatives tear substitutes goblet cells mouse Hyaluronic acid medicine Animals Hyaluronic Acid Coloring Agents Staining and Labeling Secretory Vesicles Mucin Mucins Epithelial Cells General Medicine Periodic Acid-Schiff Reaction Sensory Systems Microscopy Electron Ophthalmology Ophthalmic solutions medicine.anatomical_structure chemistry Alcian Blue Goblet Cells Ophthalmic Solutions |
| Zdroj: | Ophthalmic Research. 39:265-275 |
| ISSN: | 1423-0259 0030-3747 |
| DOI: | 10.1159/000108120 |
| Popis: | Aim: The effects of the administration of three tear substitutes on normal conjunctival epithelium of the mouse, with particular regard to goblet cells, were studied. Methods: Three-month-old Swiss CD 1 mice were divided into four groups of 7 animals each. Group 1 was untreated (control). The other animals were treated with the instillation of 5 drops/day for 10 days as follows: group 2, 0.2% sodium hyaluronate (SH); group 3, 0.5% hydroxypropylmethylcellulose (HPMC); group 4, 0.9% NaCl. The entire conjunctiva was processed for light and electron microscopy. A morphometric study was performed to evaluate: the number and size of goblet cells and the number, size and optical density of mucin granules. Furthermore, Alcian blue/periodic acid-Schiff (AB/PAS) staining for goblet cells was carried out. Results: The SH-treated group showed that the area and number of goblet cells were similar to the untreated group; the mucin granules were reduced in number and optical density but increased in size. The HPMC-treated group showed a reduced area of goblet cells with reduced optical density of mucin granules. The saline-treated group showed that goblet cells were reduced in area and number; mucin granules showed a reduction in number and optical density and an increased size. The AB/PAS stain showed that each treatment was able to induce a treatment-specific modification of the staining characteristics of goblet cells. Conclusion: Our results strongly suggest that tear substitutes can induce treatment-specific modifications of the conjunctival epithelium with particular regard to goblet cell structure and ultrastructure. |
| Databáze: | OpenAIRE |
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