Tumour necrosis factor-alpha promoter polymorphism and its association with viral dilated cardiomyopathy in Indian population: A pilot study
| Autor: | M. Sharma, Uma Nahar Saikia, Ajay Bahl, Baijayantimala Mishra, Subhabrata Sarkar, Radha K. Ratho |
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| Rok vydání: | 2015 |
| Předmět: |
Cardiomyopathy
Dilated Microbiology (medical) Genotyping Techniques lcsh:QR1-502 Cardiomyopathy India Pilot Projects Single-nucleotide polymorphism Biology Polymerase Chain Reaction Polymorphism Single Nucleotide lcsh:Microbiology polymorphism Genotype medicine Adenovirus Humans Promoter Regions Genetic enterovirus Tumor Necrosis Factor-alpha Promoter Dilated cardiomyopathy medicine.disease Virology dilated cardiomyopathy Virus Diseases TNF-α Immunology Tumor necrosis factor alpha Gene polymorphism Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Indian Journal of Medical Microbiology, Vol 33, Iss 1, Pp 16-20 (2015) |
| ISSN: | 0255-0857 |
| Popis: | Purpose: Tumour necrosis factor-α (TNF-α), a pro-inflammatory cytokine has been implicated in the pathophysiology of several viral infections. TNF-α promoter gene polymorphism is thus believed to play the modulating role in this disease pathogenesis. Several studies have shown the increased level of TNF-α in dilated cardiomyopathy (DCM). However, the role of the TNF-α promoter polymorphism is yet to be delineated in this regard. The present study for the first time tried to explore the association of TNF-α gene polymorphism with DCM of viral aetiology. Materials and Methods: Eighteen histopathologically proven DCM cases with viral genome positivity and 17 healthy controls were genotyped using polymerase chain reaction of TNF-α promoter gene followed by restriction fragment length polymorphism to determine the SNPs of -238G/A, -308G/A, -857C/T and -863C/A. Results: Of the 18 DCM cases 4 (22.2%) were positive for adenovirus (AdV), 2 (11.1%) for enterovirus (EV) and 12 (66.7%) had co-infection. Six of the 18 DCM cases (35.3%) had -238G/A polymorphism, and 10 (55.5%) had -863 homozygous AA genotype. The association of these polymorphisms was statistically significant as compared to controls (P < 0.05). Conclusions: The present pilot study suggests the possible association of TNFα -238G/A and -863C/A polymorphism with DCM of viral aetiology. |
| Databáze: | OpenAIRE |
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