Exosomal long noncoding RNA lnc-GNAQ-6:1 may serve as a diagnostic marker for gastric cancer
| Autor: | Shibao Li, Yinhai Xu, Peiming Zheng, Haoliang Zhang, Miaomiao Zhang, Kan Hu, Ping Ma, Chenchen Cai, Jiwei Wang, Lubin Shi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Clinical Biochemistry Exosomes Biochemistry Exosome Metastasis 03 medical and health sciences 0302 clinical medicine Carcinoembryonic antigen Stomach Neoplasms Biomarkers Tumor Humans Medicine Aged Oligonucleotide Array Sequence Analysis Aged 80 and over Receiver operating characteristic biology business.industry Gene Expression Profiling Biochemistry (medical) Cancer General Medicine Middle Aged medicine.disease Long non-coding RNA Microvesicles 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Cancer research Biomarker (medicine) Female RNA Long Noncoding business |
| Zdroj: | Clinica Chimica Acta. 501:252-257 |
| ISSN: | 0009-8981 |
| Popis: | Background Gastric cancer (GC) is one of the most aggressive cancers, with limited early diagnostic measures. Tumor-originated exosomal molecules are regarded as suitable candidates for non-invasive diagnosis. This study aimed to investigate the capacity of exosomal long noncoding RNA lnc-GNAQ-6:1 as a biomarker for early diagnosis of GC. Methods In this study, we collected sera from 43 patients with gastric cancer and 27 healthy subjects, then exosomes were isolated using commercial kits. Particle size analysis, Western bloting and protein-based exosomes quantification were conducted to identify the isolated exosomes and to evaluate its yield and purity. Expression levels of exosomal lnc-GNAQ-6:1 were detected by quantitative reverse transcription PCR (qRT-PCR). The serum concentrations of traditional biomarker (CA72-4, CEA, and CA19-9) were measured via a chemiluminescent detection system.The receiver operating characteristic curve (ROC) and area under curve (AUC) were used to estimate the diagnostic capacity. Furthermore, we analyzed the potential relationship between serum exosomal lnc-GNAQ-6:1 expression and clinicopathological parameters of gastric cancer. Results The exosomes extracted in this study exhibited the typical exosome characteristics and purity. Patients with gastric cancer had the higher exosome yield than healthy volunteer. The results of qRT-PCR showed that compared with the healthy control, the expression of lnc-GNAQ-6:1 was significantly lower in the gastric cancer group. The area under the ROC curve is 0.732, which was higher than the diagnostic accuracy of CEA, CA 19–9 and CA72–4. However, the expression level of lnc-GNAQ-6:1 was not correlated with gender, age, tumor metastasis, serum carcinoembryonic antigen (CEA), carbohydrate antigen 19–9 (CA19-9), and carbohydrate antigen 72–4(CA72-4). Conclusions Our data demonstrated that serum exosomal lnc-GNAQ-6:1 is lowly expressed in patient with gastric cancer and might be evaluated in larger studies as a new diagnostic marker for gastric cancer. |
| Databáze: | OpenAIRE |
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