Transcriptome Analysis Reveals Multiple Hormones, Wounding and Sugar Signaling Pathways Mediate Adventitious Root Formation in Apple Rootstock

Autor: Caiping Zhao, Jiangping Mao, Feng Dong, Liu Zhen, Yuan Meng, Lu Bao, Mingyu Han, Libo Xing, Ke Li, Yongqi Liang, Dong Zhang
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Candidate gene
Indoles
Endogeny
adventitious root (AR)
Plant Roots
01 natural sciences
lcsh:Chemistry
Transcriptome
Plant Growth Regulators
Gene Expression Regulation
Plant
sugar metabolism
lcsh:QH301-705.5
Spectroscopy
Plant Proteins
chemistry.chemical_classification
Plant Stems
General Medicine
Cell cycle
Computer Science Applications
Cell biology
apple rootstock
Malus
cell cycle
Signal transduction
education
Biology
indole-3-butyric acid (IBA)
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Auxin
Physical and Theoretical Chemistry
Molecular Biology
Gene
hormones
Sequence Analysis
RNA
Gene Expression Profiling
Organic Chemistry
wounding
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Sugars
transcriptome
010606 plant biology & botany
Hormone
Zdroj: International Journal of Molecular Sciences
Volume 19
Issue 8
International Journal of Molecular Sciences, Vol 19, Iss 8, p 2201 (2018)
ISSN: 1422-0067
Popis: Adventitious roots (AR) play an important role in the vegetative propagation of apple rootstocks. The potential role of hormone, wounding, and sugar signalling pathways in mediating AR formation has not been adequately explored and the whole co-expression network in AR formation has not been well established in apple. In order to identify the molecular mechanisms underlying AR formation in &lsquo
T337&rsquo
apple rootstocks, transcriptomic changes that occur during four stages of AR formation (0, 3, 9 and 16 days) were analyzed using high-throughput sequencing. A total of 4294 differentially expressed genes were identified. Approximately 446 genes related to hormones, wounding, sugar signaling, root development, and cell cycle induction pathways were subsequently selected based on their potential to be involved in AR formation. RT-qPCR validation of 47 genes with known functions exhibited a strong positive correlation with the RNA-seq data. Interestingly, most of the candidate genes involved in AR formation that were identified by transcriptomic sequencing showed auxin-responsive expression patterns in an exogenous Indole-3-butyric acid (IBA)-treatment assay: Indicating that endogenous and exogenous auxin plays key roles in regulating AR formation via similar signalling pathways to some extent. In general, AR formation in apple rootstocks is a complex biological process which is mainly influenced by the auxin signaling pathway. In addition, multiple hormones-, wounding- and sugar-signaling pathways interact with the auxin signaling pathway and mediate AR formation in apple rootstocks.
Databáze: OpenAIRE
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