Endoglucanase activity of cellulolytic bacteria from lake sediments and its application in hydrophyte degradation
| Autor: | Xu Dong, Zhenbin Wu, Mingzhen Zhang, Qianzheng Li, Yuqing Zhao, Hongpei Zhang, Qiaohong Zhou |
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| Rok vydání: | 2020 |
| Předmět: |
Geologic Sediments
Cellulase 010501 environmental sciences 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Aquatic plant Botany Genetics Lignin Organic matter Micromonospora Cellulose Molecular Biology Soil Microbiology 030304 developmental biology 0105 earth and related environmental sciences chemistry.chemical_classification 0303 health sciences biology Bacteria fungi Hydrilla food and beverages Plants biology.organism_classification Lakes chemistry biology.protein |
| Zdroj: | FEMS microbiology letters. 367(24) |
| ISSN: | 1574-6968 |
| Popis: | Hydrophytes are plants that grow in or on water. Their overgrowth adversely affects the ecosystem because of crowding out other aquatic organisms and polluting the environment with plant residue. In principle, cellulolytic bacteria can be used to degrade hydrophyte biomass. We here isolated and characterized four cellulolytic bacterial strains from Lake Donghu sediments (Wuhan, China) that are rich in organic matter and plant residues. The isolates (WDHS-01 to 04) represent Bacillus, Micromonospora and Streptomyces genera. The bacteria exhibited pronounced endoglucanase activities (from 0.022 to 0.064 U mL–1). They did not extensively degrade the emerged plant Canna indica L. However, in an Hydrilla verticillata (submerged plant) degradation medium, strain WDHS-02 exhibited a high degradation rate (54.91%), endoglucanase activity of 0.35 U mL–1 and the conversion rate of cellulose to reducing sugars of 7.15%. Correlation analysis revealed that bacterial endoglucanase activity was significantly correlated with the degradation rate, and acid detergent lignin, ash and cellulose content of the residual H. verticillata powder. In conclusion, the identified bacteria efficiently decomposed submerged plants without the need for acid–base pretreatment. They expand the set of known cellulolytic bacteria and can be used for natural degradation of submerged plants. |
| Databáze: | OpenAIRE |
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