Influence of polar solvents on permeability, stiffness and collagen dissociation of demineralized dentin

Autor: Leo Tjäderhane, Murat Mutluay, Arzu Tezvergil-Mutluay, Anas Aaqel Salim Salim Al-Ani
Přispěvatelé: Department of Oral and Maxillofacial Diseases, Clinicum, HUS Head and Neck Center
Rok vydání: 2019
Předmět:
Zdroj: International Journal of Adhesion and Adhesives. 89:148-153
ISSN: 0143-7496
DOI: 10.1016/j.ijadhadh.2019.01.003
Popis: The purpose of this in vitro study was to evaluate and compare the effect of dimethyl sulfoxide (DMSO) or ethanol on the permeability, stiffness and collagen dissociation of demineralized dentin. Dentin cubes (2 x 2 x 2 mm) were demineralized in EDTA and incubated in DMSO or ethanol (0.01, 0.1, 1, 5, 10, 20, 50 and 100%) (n = 10/group) for 30s, followed by 100% HEMA incubation. Extracted HEMA was quantified. For elastic modulus (E), demineralized dentin beams (6 x 2 x 1 mm) were incubated in DMSO or ethanol (1, 10, 20, 50 and 100%) for 10, 30 or 60 min at 3-point bending. Additional demineralized dentin discs (1 mm) were incubated in DMSO (1, 10, 50 and 100%) for 10, 30 and 60 min and the optical clearing effect was observed. The data were analyzed using ANOVA and Tukey's test (alpha = 0.05) using SigmaPlot (Systat Software Inc., San Jose, CA). Compared to controls, HEMA uptake was significantly higher with all DMSO concentrations, and with 0.1% or higher ethanol concentrations (p
Databáze: OpenAIRE