Myelin protein Po as a potential autoantigen in autoimmune inner ear disease
| Autor: | Vincent Dupriez, Jean-Paul Tomasi, Guy G. Rousseau, Naima Deggouj, Ming Y. Cao, Michel Gersdorff, Mark H. Rider |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Pathology medicine.medical_specialty Guinea Pigs Molecular Sequence Data Ear Middle Biology medicine.disease_cause Autoantigens Peptide Mapping Biochemistry Autoimmune Diseases Autoimmunity Myelin Antigen otorhinolaryngologic diseases Genetics medicine Animals Humans Inner ear Amino Acid Sequence Ear Diseases Molecular Biology Cochlea Autoantibody Autoimmune inner ear disease medicine.disease medicine.anatomical_structure Organ of Corti Female sense organs Myelin P0 Protein Biotechnology |
| Zdroj: | The FASEB Journal. 10:1635-1640 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fasebj.10.14.9002556 |
| Popis: | We have previously shown that a 30,000 Mr protein extracted from guinea pig inner ear tissue is recognized by autoantibodies present in the serum of patients suffering from autoimmune inner ear disease. This protein was localized in the modiolus and in the organ of Corti. We have now identified this protein by a combination of microsequencing and matrix-assisted laser desorption ionization time-of-flight mass spectrometry of its tryptic peptides. A partial sequence of the protein was thereby determined. These data and 2-dimensional gel electrophoresis followed by immunoblotting experiments showed that the 30,000 Mr inner ear antigen is the major peripheral myelin protein Po. This suggests that protein Po may be an important autoantigen in autoimmune inner ear disease. |
| Databáze: | OpenAIRE |
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