Pesticide mediated oxidative stress induces genotoxicity and disrupts chromatin structure in fenugreek (Trigonella foenum - graecum L.) seedlings

Autor: Sujit Roy, Samrat Banerjee, Sayanti De, Kalyan Mahapatra
Rok vydání: 2018
Předmět:
Chlorophyll
Insecticides
Health
Toxicology and Mutagenesis
0211 other engineering and technologies
02 engineering and technology
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Plant Roots
Antioxidants
Anthocyanins
Thiabendazole
Micrococcal Nuclease
Waste Management and Disposal
biology
Chemistry
Pollution
Chromatin
Fungicide
Horticulture
Phytotoxicity
Comet Assay
Genome
Plant
Environmental Engineering
Mitotic index
Trigonella
Cell Survival
Germination
Genes
Plant
medicine
Environmental Chemistry
0105 earth and related environmental sciences
021110 strategic
defence & security studies
Superoxide Dismutase
Cell Membrane
Hydrogen Peroxide
Pesticide
biology.organism_classification
Carotenoids
Fungicides
Industrial
Oxidative Stress
Thiazoles
Microscopy
Fluorescence
Seedling
Seedlings
Lipid Peroxidation
Reactive Oxygen Species
Oxidative stress
Genotoxicity
DNA Damage
Zdroj: Journal of hazardous materials. 369
ISSN: 1873-3336
Popis: Here we report cytototoxic and genotoxic potentials of four commonly used pesticides, including, tricyclazole, thiabendazole (fungicides), plethora and slash-360 (insecticides) in the non-target tropical crop plant Trigonella foenum - graecum L. (fenugreek). Three different concentrations of the selected pesticides were used. For fungicides, 0.05% and for insecticides, 0.1% concentration represents recommended doses, while, 2X and 4X concentrations of the recommended dose were used to test their phytotoxic effects. Inhibition of germination and seedling growth were clearly observed at 4X concentration of the pesticides. Tricyclazole and plethora showed more pronounced effects than the other two agrochemicals. The pesticides, particularly at 4X concentrations clearly induced oxidative stress and cytotoxic effects in Trigonella seedlings with appreciable reduction in mitotic index, induction of chromosomal abnormalities in root meristematic cell and decreased level of accumulation of some key cell cycle regulators, including CDK1, CDK2 and Cyclin B1.Detection of accumulation of DNA double strand breaks and histone H2AX phosphorylation in pesticide treated seedlings have revealed direct genotoxic effects of the selected pesticides. Overall, our results provide insights into the mechanism of pesticide induced cytotoxic and genotoxic effects in plant genome with future implications for designing pesticides to minimize their deleterious effects on non-target crop plants.
Databáze: OpenAIRE
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