Contribution of a voltage-sensitive calcium release mechanism to contraction in cardiac ventricular myocytes
| Autor: | Jiequan Zhu, Gregory R. Ferrier, Susan E. Howlett |
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| Předmět: |
Male
medicine.medical_specialty Contraction (grammar) Time Factors Calcium Channels L-Type Nifedipine Physiology Heart Ventricles Guinea Pigs chemistry.chemical_element Calcium Membrane Potentials Physiology (medical) Internal medicine medicine Myocyte Animals Cells Cultured Ryanodine receptor Ryanodine Endoplasmic reticulum Cardiac muscle Lidocaine Calcium Channel Blockers Myocardial Contraction medicine.anatomical_structure Endocrinology chemistry Verapamil Circulatory system Biophysics Liberation Thapsigargin Calcium Channels Cardiology and Cardiovascular Medicine Sodium Channel Blockers |
| Zdroj: | Europe PubMed Central |
| Popis: | The contribution of a voltage-sensitive release mechanism (VSRM) for sarcoplasmic reticulum (SR) Ca2+ to contraction was investigated in voltage-clamped ventricular myocytes at 37°C. Na+ current was blocked with lidocaine. The VSRM exhibited steady-state inactivation (half-inactivation voltage: −47.6 mV; slope factor: 4.37 mV). When the VSRM was inactivated, contraction-voltage relationships were proportional to L-type Ca2+current ( I Ca-L). When the VSRM was available, the relationship was sigmoidal, with contractions independent of voltage positive to −20 mV. VSRM and I Ca-Lcontractions could be separated by activation-inactivation properties. VSRM contractions were extremely sensitive to ryanodine, thapsigargin, and conditioning protocols to reduce SR Ca2+ load. I Ca-Lcontractions were less sensitive. When both VSRM and I Ca-L were available, sigmoidal contraction-voltage relationships became bell-shaped with protocols to reduce SR Ca2+ load. Myocytes demonstrated restitution of contraction that was slower than restitution of I Ca-L. Restitution was a property of the VSRM. Thus activation and recovery of the VSRM are important in coupling cardiac contraction to membrane potential, SR Ca2+ load, and activation interval. |
| Databáze: | OpenAIRE |
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