Black pepper constituent piperine: Genotoxicity studies in vitro and in vivo
| Autor: | Tina Woehrle, Ankie Schoenmakers, Anette Thiel, Markus Fehr, Carin Buskens, Paul Beilstein, Stephane Etheve |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Polyunsaturated Alkamides Spleen CHO Cells In Vitro Techniques Pharmacology Toxicology medicine.disease_cause Chromosome aberration Mice chemistry.chemical_compound Alkaloids Cricetulus Piperidines In vivo Cricetinae medicine Animals Benzodioxoles Mutagenicity Tests Chemistry General Medicine In vitro medicine.anatomical_structure Piperine Micronucleus test Female Piper nigrum Micronucleus Genotoxicity Food Science |
| Zdroj: | Food and Chemical Toxicology. 66:350-357 |
| ISSN: | 0278-6915 |
| Popis: | Piperine is responsible for the hot taste of black pepper. Publications on genotoxicity of piperine are reported: negative Ames Tests and one in vitro micronucleus test (MNT). In vivo tests were mainly negative. In the majority of the data the administered dose levels did not follow the dose selection requirements of regulatory guidelines of having dose levels up to the maximum tolerated dose (MTD). The only oral high dose studies were a positive in vivo MNT in mice in contrast to a negative in vivo chromosome aberration test in rats. Thus, conflicting results in genotoxicity testing are published. To investigate this further, we administered piperine to mice up to the MTD and determined micronuclei-frequency. Piperine reduces core body temperature and interferes with blood cells both being known to result in irrelevant positive in vivo MNTs. Therefore we added mechanistic endpoints: core body temperature, haematology, erythropoietin level, and organ weights. Additionally an in vitro MNT in Chinese hamster ovary cells was performed. Piperine was negative in the in vitro MNT. It caused significant reduction of core body temperature, decrease of white blood cells and spleen weights but no increase in the micronucleus-frequency. Thus, in our studies piperine was not genotoxic. |
| Databáze: | OpenAIRE |
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