Differences in Intestinal Gene Expression Profiles in Broiler Lines Varying in Susceptibility to Malabsorption Syndrome
| Autor: | Johanna M.J. Rebel, A.J.W. Hoekman, M.A. Smits, S. van Hemert |
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| Rok vydání: | 2004 |
| Předmět: |
disease resistance
Malabsorption Microarray chicken mareks-disease Down-Regulation Animal Breeding and Genomics Plant disease resistance Biology reovirus Malabsorption Syndromes generation Complementary DNA Gene expression medicine Animals Fokkerij en Genomica Gene Poultry Diseases DNA Primers Gene Library Oligonucleotide Array Sequence Analysis Expressed Sequence Tags Genetics Reverse Transcriptase Polymerase Chain Reaction Microarray analysis techniques Gene Expression Profiling fungi Broiler Sequence Analysis DNA General Medicine medicine.disease Molecular biology Jejunum Gene Expression Regulation Intestinal Absorption WIAS impact Hybridization Genetic Animal Science and Zoology Chickens ID - Dier en Omgeving programs cdna microarray |
| Zdroj: | Poultry Science, 83(10), 1675-1682 Europe PubMed Central Poultry Science 83 (2004) 10 |
| ISSN: | 0032-5791 |
| DOI: | 10.1093/ps/83.10.1675 |
| Popis: | Examination of the host gene expression response upon encounters with pathogens may provide insights into the cellular events following an infection. In addition, it may shed light on the basic mechanisms underlying differences in the susceptibility of the host. In this study gene expression in the chicken jejunum was investigated in 2 different broiler lines under control and malabsorption syndrome (MAS) affected conditions. The 2 broiler lines differ in their susceptibility for MAS. The gene expression was investigated at 6 different times post-inoculation using a custom-made intestine specific cDNA microarray. More than 70 up- or downregulated genes were identified after a MAS inoculation in both broiler lines. However, the number of the up- and downregulated genes varied between the 2 lines, with more differences in expression in the most susceptible line. Marked differences were observed in expression profiles between the 2 broiler lines, in control as well as in the MAS affected birds. The microarray data were validated and confirmed by quantitative real time PCR. The differentially expressed genes included immune related genes, genes associated with food absorption, and genes that need to be characterized further before their role in MAS and MAS susceptibility can be understood. |
| Databáze: | OpenAIRE |
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