Cell-free DNA in a three-dimensional spheroid cell culture model: A preliminary study
| Autor: | Abel Jacobus Bronkhorst, Sias Hamman, Janine Aucamp, Krzysztof Wrzesinski, Piet J. Pretorius, Carlemi Calitz, Chrisna Gouws |
|---|---|
| Přispěvatelé: | 20505698 - Aucamp, Janine, 22195289 - Bronkhorst, Abel Jacobus, 10176705 - Pretorius, Petrus Jacobus, 12450960 - Gouws, Chrisna, 20743149 - Calitz, Carlemi, 10081097 - Hamman, Josias Hendrik |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Rotating bioreactors Biology Biochemistry Capillary electrophoresis 03 medical and health sciences 3D cell culture chemistry.chemical_compound Cell-free DNA 0302 clinical medicine In vivo Spheroids Cellular Humans Growth medium Spheroid Cell Biology Hep G2 Cells Molecular biology Cell biology 030104 developmental biology Glucose Cell-free fetal DNA chemistry Cell culture 030220 oncology & carcinogenesis D cell culture Sample collection Cell culture model Cell-Free Nucleic Acids |
| Zdroj: | Aucamp, J, Calitz, C, Bronkhorst, A J, Wrzesinski, K, Hamman, S, Gouws, C & Pretorius, P J 2017, ' Cell-free DNA in a three-dimensional spheroid cell culture model : A preliminary study ', International Journal of Biochemistry and Cell Biology, vol. 89, pp. 182-192 . https://doi.org/10.1016/j.biocel.2017.06.014 |
| ISSN: | 1878-5875 |
| DOI: | 10.1016/j.biocel.2017.06.014 |
| Popis: | Background Investigating the biological functions of cell-free DNA (cfDNA) is limited by the interference of vast numbers of putative sources and causes of DNA release into circulation. Utilization of three-dimensional (3D) spheroid cell cultures, models with characteristics closer to the in vivo state, may be of significant benefit for cfDNA research. Methods CfDNA was isolated from the growth medium of C3A spheroid cultures in rotating bioreactors during both normal growth and treatment with acetaminophen. Spheroid growth was monitored via planimetry, lactate dehydrogenase activity and glucose consumption and was related to isolated cfDNA characteristics. Results Changes in spheroid growth and stability were effectively mirrored by cfDNA characteristics. CfDNA characteristics correlated with that of previous two-dimensional (2D) cell culture and human plasma research. Conclusions 3D spheroid cultures can serve as effective, simplified in vivo-simulating “closed-circuit” models since putative sources of cfDNA are limited to only the targeted cells. In addition, cfDNA can also serve as an alternative or auxiliary marker for tracking spheroid growth, development and culture stability. Biological significance 3D cell cultures can be used to translate “closed-circuit” in vitro model research into data that is relevant for in vivo studies and clinical applications. In turn, the utilization of cfDNA during 3D culture research can optimize sample collection without affecting the stability of the growth environment. Combining 3D culture and cfDNA research could, therefore, optimize both research fields. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect