Internalization of hydroxyapatite nanoparticles in liver cancer cells
| Autor: | Ingo Werner Bauer, Lin Yuan, Shipu Li, Ying-Chao Han, Meizhen Yin |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Materials science
Carcinoma Hepatocellular Biocompatibility media_common.quotation_subject Biomedical Engineering Biophysics Nanoparticle Bioengineering Coated Pit Nanotechnology Endocytosis Biomaterials Cell membrane Drug Delivery Systems stomatognathic system Microscopy Electron Transmission medicine Tumor Cells Cultured Humans Scattering Radiation Particle Size Internalization media_common Lasers Liver Neoplasms Coated Pits Cell-Membrane Clathrin medicine.anatomical_structure Durapatite Cell culture Cancer cell Potassium Nanoparticles |
| Zdroj: | Journal of materials science. Materials in medicine. 19(3) |
| ISSN: | 0957-4530 |
| Popis: | Hydroxyapatite (HAP) is the main inorganic component of hard tissues and shows excellent biocompatibility and osteoconductivity properties. Nanoparticles of HAP can be synthesised by the precipitation method in distilled water. The needle shaped particles are below 100 nm in size with low-crystallinity and high-surfacial activation. Recent studies showed toxic effects of HAP nanoparticles on cancer cells. Other studies focus on the application of HAP nanoparticles as drug and gene delivery system or cell marker. However, to date, the exact internalization pathway of HAP nanoparticles into cells has not been determined. When HAP nanoparticles were added to cell culture medium, the particles immediately became instable and formed agglomerates with a size of about 500-700 nm. Hence, cells seldom encounter single HAP nanoparticles in the environment of cell culture or body fluid. The TEM showed internalized HAP captured by vacuoles in the cytoplasm of the hepatocellular carcinoma cells. The invaginations in the cell membrane before nanoparticle uptake suggested endocytic pathways as internalization mechanism. This study revealed that agglomerated HAP nanoparticles were internalized by cells through the energy-dependent process of clathrin-mediated endocytosis. Depletion of intracellular potassium arrested the formation of coated pit, which inhibited the uptake of HAP. |
| Databáze: | OpenAIRE |
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