A MICROSPECTROFLUOROMETER WITH EPI-ILLUMINATION OPERATED UNDER COMPUTER CONTROL
| Autor: | J. A. De Sterke, H. Wasmund, J. S. Ploem, J. Bonnet |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Time Factors
Histology Materials science Analytical chemistry Iris law.invention Norepinephrine Optics law Methods Animals Epi illumination Monochromator Digital computer Autoanalysis Computers Histocytochemistry business.industry Fluorescence Line (electrical engineering) Rats Intensity (physics) Computer control Microscopy Fluorescence Evaluation Studies as Topic Anatomy business Excitation |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 22:668-677 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/22.7.668 |
| Popis: | Until recently the recording of fully corrected excitation and fluorescence spectra from fluorophores in microscopic preparations has been a time-consuming procedure. A new type of microspectrofluorometer with epi-illumination has been developed. This instrument is operated on line with a digital computer permitting automatic operation of monochromator motors, electrical shutters and correction of spectra. The electrical shutters are used for a rapid change between low intensity dia- and strong epi-illumination. Exposure to epi-illumination is limited to the short period of recording the spectra. A new optical system permits centering of structures to be measured, using the binocular head for viewing the illuminated image of the monochromator slit and the fluorescent image excited with dia-illumination simultaneously. New objectives and eyepieces with a high light collecting power permit the visualization of weak fluorescence obtained with low intensity dia-illumination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|