Selenium-Cultured Potamogeton maackianus in the Diet Can Alleviate Oxidative Stress and Immune Suppression in Chinese Mitten Crab (Eriocheir sinensis) Under Copper Exposure
| Autor: | Hao-Jun Zhu, Chuan-Kun Zhu, Jun Qiang, Xue-Jun Duan, Yi-Fan Tao, Jie He, Pao Xu, Jin-Wen Bao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
Physiology medicine.medical_treatment Glutathione reductase lcsh:Physiology Potamogeton maackianus 03 medical and health sciences chemistry.chemical_compound Animal science Physiology (medical) medicine oxidative stress selenium 030304 developmental biology Original Research chemistry.chemical_classification Chinese mitten crab 0303 health sciences biology lcsh:QP1-981 Glutathione peroxidase immune regulation food and beverages 04 agricultural and veterinary sciences biology.organism_classification Malondialdehyde Cu2+ exposure Eriocheir chemistry Catalase 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Hepatopancreas Chinese mitten crab (Eriocheir sinensis) |
| Zdroj: | Frontiers in Physiology Frontiers in Physiology, Vol 11 (2020) |
| ISSN: | 1664-042X |
| Popis: | Selenium (Se) is an essential trace element for aquatic animals. The aquatic plant Potamogeton maackianus is an important natural food of Chinese mitten crab (Eriocheir sinensis). The aim of this study was to determine whether the antioxidant and immune responses of Chinese mitten crab are affected by including Se-cultured P. maackianus in the diet. Three groups of P. maackianus were cultured at levels of 0.02 mg/kg Se, 8.83 mg/kg Se, and 16.92 mg/kg Se, and the plants in these groups were used in experimental diets fed to crabs (dietary Se content of 0.05, 0.43, and 0.82 mg/kg, respectively). Compared with crabs in the 0.05 mg/kg group, those in the 0.82 mg/kg group showed significantly increased specific growth rate, protease and lipase activities, triglyceride and cholesterol contents, and Se content in the hepatopancreas and muscle (P < 0.05); increased activities of glutathione peroxidase, glutathione reductase, and catalase in the antioxidant system; increased transcript levels of MT (encoding metallothionein); and decreased malondialdehyde content (P < 0.05). At the end of the rearing experiment, the crabs in the different groups were exposed to copper (Cu2+) stress for 96 h. All the juvenile crabs in the 0.43 and 0.82 mg/kg groups survived 96 h of Cu2+ stress. Crabs in the 0.82 mg/kg group showed enhanced antioxidant responses under Cu2+ stress, increased transcript levels of MT and LYZ, and increased resistance. Therefore, supplementation of the diet of Chinese mitten crab with increased levels of Se-cultured P. maackianus can reduce oxidative stress under Cu2+ exposure, activate the immune response, and benefit growth. |
| Databáze: | OpenAIRE |
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