Cadmium activates ZmMPK3-1 and ZmMPK6-1 via induction of reactive oxygen species in maize roots
| Autor: | Tianshuai Xia, Jianhua Qi, Lixia Liu, Yukun Liu, Peiyu Dang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
inorganic chemicals
0301 basic medicine MAPK/ERK pathway MAP Kinase Signaling System Immunoprecipitation Immunoblotting Biophysics chemistry.chemical_element Plant Roots Zea mays Biochemistry Gene Expression Regulation Enzymologic 03 medical and health sciences 0302 clinical medicine Cadmium Chloride Immunoblot Analysis Protein kinase A Molecular Biology Plant Proteins chemistry.chemical_classification Reactive oxygen species Cadmium Kinase Cell Biology Cell biology Enzyme Activation Isoenzymes MAPK activation 030104 developmental biology chemistry 030220 oncology & carcinogenesis Mitogen-Activated Protein Kinases Reactive Oxygen Species |
| Zdroj: | Biochemical and Biophysical Research Communications. 516:747-752 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2019.06.116 |
| Popis: | Both mitogen-activated protein kinase (MAPK) cascades and reactive oxygen species (ROS) are critical to signaling in eukaryotes. Cadmium induces MAPK activation and ROS production in plants. This study aims to identify specific MAPKs activated by CdCl2 in maize roots, and examine the relationship between MAPK activation and ROS production under CdCl2 treatment. Using in-gel kinase assays, immunoprecipitation, and immunoblot analysis, we identified 43 and 45 kDa ERK-like MAPKs in response to CdCl2. Further analysis revealed that ZmMPK3-1 and ZmMPK6-1 correspond to the 43 and 45 kDa MAPKs, respectively. In addition, CdCl2 induced ROS production prior to the activation of ZmMPK3-1 and ZmMPK6-1. Inhibition of ROS attenuated Cd-activation of ZmMPK3-1 and ZmMPK6-1, whereas inhibition of MAPK signaling did not disturb Cd-induced ROS production. Collectively, these results indicate that, in maize roots, cadmium stress activates ZmMPK3-1 and ZmMPK6-1 via ROS induction. |
| Databáze: | OpenAIRE |
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