Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin
| Autor: | Nathalia V. N. da Paz, Renata Angeli, Luiz Felipe Pereira Borba Carvalho, Ana Paula Valente, Maria Tereza dos Santos Correia, Fabio C. L. Almeida, Maria da Paz Carvalho da Silva, Flavia F. B. Araújo, Luana Cassandra Breitenbach Barroso Coelho, Glícia Maria Torres Calazans, J. C. Maciel, Patrícia Maria Guedes Paiva |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Article Subject
lcsh:Biotechnology Health Toxicology and Mutagenesis Ion chromatography lcsh:Medicine Affinity chromatography lcsh:TP248.13-248.65 Genetics Concanavalin A Molecular Biology Nuclear Magnetic Resonance Biomolecular Zymomonas Chromatography biology Elution Chemistry lcsh:R Lectin Fabaceae General Medicine Hemagglutination Inhibition Tests biology.organism_classification Canavalia Ferrosoferric Oxide Fructans Biochemistry Sephadex Canavalia ensiformis biology.protein Molecular Medicine Plant Lectins Biotechnology Research Article Chromatography Liquid |
| Zdroj: | Journal of Biomedicine and Biotechnology Journal of Biomedicine and Biotechnology, Vol 2009 (2009) |
| ISSN: | 1110-7243 |
| DOI: | 10.1155/2009/179106 |
| Popis: | A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field. Lectins fromCanavalia ensiformis(Con A) andCratylia mollis(Cramoll 1 and Cramoll 1,4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column. |
| Databáze: | OpenAIRE |
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