Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation
Autor: | Martin J. Hoogduijn, Carla C. Baan, L.J.W. van der Laan, Wenda Verschoor, Robert C. Minnee, Jeroen G. H. P. Verhoeven, Dennis A. Hesselink, M. W. F. van den Hoogen, H. Tejeda-Mora, Karin Boer |
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Přispěvatelé: | Internal Medicine, Surgery |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Adult
Graft Rejection Male 0301 basic medicine Nephrology medicine.medical_specialty Pathology Time Factors Science Human leukocyte antigen 030230 surgery Article 03 medical and health sciences Medical research 0302 clinical medicine Internal medicine Biopsy medicine Humans Hepatitis A Virus Cellular Receptor 1 Kidney transplantation Kidney Multidisciplinary medicine.diagnostic_test business.industry Endothelial Cells Middle Aged Allografts Flow Cytometry medicine.disease Kidney Transplantation Phenotype Transplantation 030104 developmental biology medicine.anatomical_structure surgical procedures operative cardiovascular system Biomarker (medicine) Medicine Female business Biomarkers |
Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) Scientific Reports, 11(1). Nature Publishing Group Scientific Reports |
ISSN: | 2045-2322 |
Popis: | The diagnosis of kidney allograft rejection is based on late histological and clinical markers. Early, specific and minimally-invasive biomarkers may improve rejection diagnosis. Endothelial cells (EC) are one of the earliest targets in kidney transplant rejection. We investigated whether circulating EC (cEC) could serve as an earlier and less invasive biomarker for allograft rejection. Blood was collected from a cohort of 51 kidney transplant recipients before and at multiple timepoints after transplantation, including during a for cause biopsy. The number and phenotype of EC was assessed by flow-cytometric analysis. Unbiased selection of EC was done using principal component (PCA) analysis. Paired analysis revealed a transient cEC increase of 2.1-fold on the third day post-transplant, recovering to preoperative levels at seventh day post-transplant and onwards. Analysis of HLA subtype demonstrated that cEC mainly originate from the recipient. cEC levels were not associated with allograft rejection, allograft function or other allograft pathologies. However, cEC in patients with allograft rejection and increased levels of cEC showed elevated levels of KIM-1 (kidney injury marker-1). These findings indicate that cEC numbers and phenotype are affected after kidney transplantation but may not improve rejection diagnosis. |
Databáze: | OpenAIRE |
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