Chemiluminescence response induced by mesenteric ischaemia/reperfusion: effect of antioxidative compounds ex vivo
| Autor: | Ružena Sotníková, Silvia Banášová, Katarína Drábiková, Jana Navarová, Silvia Fialová, Viera Nosáľová, Daniela Košťálová |
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| Rok vydání: | 2010 |
| Předmět: |
Health
Toxicology and Mutagenesis Radical Ischemia Pharmacology Toxicology stobadine chemistry.chemical_compound In vivo Medicine arbutin biology business.industry SMe1EC2 Arbutin ischaemia/reperfusion medicine.disease chemiluminescence Enzyme assay Biochemistry chemistry Myeloperoxidase biology.protein Curcumin Original Article business Ex vivo |
| Zdroj: | Interdisciplinary Toxicology |
| ISSN: | 1337-9569 1337-6853 |
| DOI: | 10.2478/v10102-010-0021-3 |
| Popis: | Chemiluminescence response induced by mesenteric ischaemia/reperfusion: effect of antioxidative compoundsex vivoIschaemia and reperfusion (I/R) play an important role in human pathophysiology as they occur in many clinical conditions and are associated with high morbidity and mortality. Interruption of blood supply rapidly damages metabolically active tissues. Restoration of blood flow after a period of ischaemia may further worsen cell injury due to an increased formation of free radicals. The aim of our work was to assess macroscopically the extent of intestinal pathological changes caused by mesenteric I/R, and to study free radical production by luminol enhanced chemiluminescence (CL) of ileal samples. In further experiments, the antioxidative activity of the drugs tested was evaluated spectrophotometrically by the use of the DPPH radical. We studied the potential protectiveex vivoeffect of the plant origin compound arbutin as well as of the pyridoindole stobadine and its derivative SMe1EC2. I/R induced pronounced haemorrhagic intestinal injury accompanied by increase of myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAGA) activity. Compared to sham operated (control) rats, there was only a slight increase of CL response after I/R, probably in association with neutrophil increase, indicated by enhanced MPO activity. All compounds significantly reduced the peak values of CL responses of the ileal samplesex vivo, thus reducing the I/R induced increase of free radical production. The antioxidants studied showed a similar inhibitory effect on the CL response influenced by mesenteric I/R. If provedin vivo, these compounds would represent potentially useful therapeutic antioxidants. |
| Databáze: | OpenAIRE |
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