Yeast Life Span Extension by Depletion of 60S Ribosomal Subunits Is Mediated by Gcn4
| Autor: | Nick Dang, Brian K. Kennedy, Matt Kaeberlein, Lindsay A. Fox, Vivian L. MacKay, Jonathan A. Oakes, Emily O. Kerr, Stanley Fields, Diana N. Pak, Mitsuhiro Tsuchiya, Bie N. Tchao, Di Hu, Elijah D. Johnston, Kristan K. Steffen |
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| Jazyk: | angličtina |
| Předmět: |
Ribosomal Proteins
Saccharomyces cerevisiae Proteins PROTEINS Protein subunit Ribosome biogenesis Saccharomyces cerevisiae Biology Histone Deacetylases Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Sirtuin 2 0302 clinical medicine Sirtuins Protein kinase A Transcription factor Silent Information Regulator Proteins Saccharomyces cerevisiae 030304 developmental biology 2. Zero hunger 0303 health sciences Biochemistry Genetics and Molecular Biology(all) Kinase Eukaryotic Large Ribosomal Subunit Ribosome Subunits Large Eukaryotic Ribosomal RNA DNA-Binding Proteins Basic-Leucine Zipper Transcription Factors Biochemistry SIGNALING CELLBIO Gene Deletion 030217 neurology & neurosurgery Biogenesis Transcription Factors |
| Zdroj: | Cell. (2):292-302 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2008.02.037 |
| Popis: | SummaryIn nearly every organism studied, reduced caloric intake extends life span. In yeast, span extension from dietary restriction is thought to be mediated by the highly conserved, nutrient-responsive target of rapamycin (TOR), protein kinase A (PKA), and Sch9 kinases. These kinases coordinately regulate various cellular processes including stress responses, protein turnover, cell growth, and ribosome biogenesis. Here we show that a specific reduction of 60S ribosomal subunit levels slows aging in yeast. Deletion of genes encoding 60S subunit proteins or processing factors or treatment with a small molecule, which all inhibit 60S subunit biogenesis, are each sufficient to significantly increase replicative life span. One mechanism by which reduced 60S subunit levels leads to life span extension is through induction of Gcn4, a nutrient-responsive transcription factor. Genetic epistasis analyses suggest that dietary restriction, reduced 60S subunit abundance, and Gcn4 activation extend yeast life span by similar mechanisms. |
| Databáze: | OpenAIRE |
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