Equilibria in DPPC-Diosgenin and DPPC-Diosgenin Acetate Bilayer Lipid Membranes: Interfacial Tension and Microelectrophoretic Studies
| Autor: | Aneta D. Petelska, Ewelina Skrodzka, Joanna Kotyńska, Katarzyna Karwowska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
liposomes
Materials science complex formation equilibria interfacial tension 02 engineering and technology Electrolyte 010402 general chemistry 01 natural sciences Surface tension Microelectrophoresis Materials Chemistry diosgenin acetate Lipid bilayer Liposome technology industry and agriculture Surfaces and Interfaces 021001 nanoscience & nanotechnology microelectrophoresis 0104 chemical sciences Surfaces Coatings and Films lipid bilayer Electrophoresis Membrane Isoelectric point Chemical engineering lcsh:TA1-2040 diosgenin lipids (amino acids peptides and proteins) DPPC 0210 nano-technology lcsh:Engineering (General). Civil engineering (General) |
| Zdroj: | Coatings Volume 10 Issue 4 Coatings, Vol 10, Iss 368, p 368 (2020) |
| ISSN: | 2079-6412 |
| DOI: | 10.3390/coatings10040368 |
| Popis: | Interactions between components of model lipid membranes (spherical lipid bilayers and liposomes) are investigated here. Parameters characterizing equilibria in the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)-diosgenin (Dio) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)-diosgenin acetate (DAc) membrane systems have been determined. The interfacial tension measurement of spherical lipid bilayers was based on the Young-Laplace&rsquo s equation using a homemade computer-controlled device. We assume a 1:1 complex in the DPPC-Dio and DPPC-DAc membrane systems. The parameters A 3 &minus 1 , the surface concentration of lipid membranes formed from these complexes, &gamma 3 , the interfacial tension of such membranes, and, K, the constant stability of these complexes were calculated. Microelectrophoresis was used for examinations of the surface charge density of lipid membranes. The values were obtained here from electrophoretic mobility data applying Smoluchowsky&rsquo s equation. The effect of pH (pH ranged of 2 to 10) on the electrolyte solution and the compositions of the membranes was analyzed. The obtained results indicate that the modification of DPPC membranes with both Dio and DAc causes changes in surface charge density values and shifts of the isoelectric point. |
| Databáze: | OpenAIRE |
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