Cytotoxicity evaluation of chlorhexidine gluconate on human fibroblasts, myoblasts, and osteoblasts
| Autor: | Jordan Werner, James X. Liu, Mandeep S. Virk, Thorsten Kirsch, Joseph D. Zuckerman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Serial dilution
Cell Andrology 03 medical and health sciences 0302 clinical medicine lcsh:Orthopedic surgery In vivo fibroblasts medicine Cytotoxic T cell Orthopedics and Sports Medicine Cytotoxicity 030222 orthopedics Chemistry chlorhexidine myoblasts Chlorhexidine osteoblasts Cell counting In vitro lcsh:RD701-811 Infectious Diseases medicine.anatomical_structure cytotoxicity Surgery 030217 neurology & neurosurgery Research Paper medicine.drug |
| Zdroj: | Journal of Bone and Joint Infection, Vol 3, Pp 165-172 (2018) Journal of Bone and Joint Infection |
| ISSN: | 2206-3552 |
| Popis: | Introduction: Chlorhexidine gluconate (CHX) is widely used as a preoperative surgical skin-preparation solution and intra-wound irrigation agent, with excellent efficacy against wide variety of bacteria. The cytotoxic effect of CHX on local proliferating cells following orthopaedic procedures is largely undescribed. Our aim was to investigate the in vitro effects of CHX on primary fibroblasts, myoblasts, and osteoblasts.Methods: Cells were exposed to CHX dilutions (0%, 0.002%, 0.02%, 0.2%, and 2%) for either a 1, 2, or 3-minute duration. Cell survival was measured using a cytotoxicity assay (Cell Counting Kit-8). Cell migration was measured using a scratch assay: a “scratch” was made in a cell monolayer following CHX exposure, and time to closure of the scratch was measured.Results: All cells exposed to CHX dilutions of ≥ 0.02% for any exposure duration had cell survival rates of less than 6% relative to untreated controls (p < 0.001). Cells exposed to CHX dilution of 0.002% all had significantly lower survival rates relative to control (p < 0.01) with the exception of 1-minute exposure to fibroblasts, which showed 96.4% cell survival (p = 0.78). Scratch defect closure was seen in < 24 hours in all control conditions. However, cells exposed to CHX dilutions ≥ 0.02% had scratch defects that remained open indefinitely.Conclusions: The clinically used concentration of CHX (2%) permanently halts cell migration and significantly reduces survival of in vitro fibroblasts, myoblasts, and osteoblasts. Further in vivo studies are required to examine and optimize CHX safety and efficacy when applied near open incisions or intra-wound application. |
| Databáze: | OpenAIRE |
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