Clinical reliability of IgG, IgA, and IgM antibodies in detecting Epstein-Barr virus at different stages of infection with a commercial nonrecombinant polyantigenic ELISA
| Autor: | María del Carmen Maroto, Gonzalo Piédrola, M. J. Vergara, José Gutiérrez |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
Epstein-Barr Virus Infections Igm antibody Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Disease Antibodies Viral medicine.disease_cause Sensitivity and Specificity Asymptomatic Virus medicine False positive paradox Humans Immunology and Allergy Child biology business.industry Biochemistry (medical) Public Health Environmental and Occupational Health Original Articles Hematology Epstein–Barr virus Virology Immunoglobulin A Medical Laboratory Technology Immunoglobulin M Child Preschool Immunoglobulin G Immunology Elisa test biology.protein medicine.symptom Antibody business |
| Zdroj: | Journal of Clinical Laboratory Analysis. 13:65-68 |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/(sici)1098-2825(1999)13:2<65::aid-jcla4>3.0.co;2-o |
| Popis: | We studied the diagnostic reliability of a modification of the Enzygnost EBV test (Behringwerke, Germany) for the detection of IgG, IgA, and IgM antibodies (Abs) in the diagnosis of Epstein‐Barr virus (EBV) disease. One hundred and twenty‐three serum samples were studied: 14 asymptomatic subjects without EBV infection, 48 patients with primary infection, 46 subjects with past EBV infection (11 patients with other acute infections), 8 patients without EBV infection but with other viral infection, and 7 patients with probable acute clonal stimulation of B lymphocytes caused by different microorganisms. Enzygnost EBV is based on an ELISA test with a pool of viral antigens. In our series the reliability of IgM for the diagnosis of recent primary EBV infection was: sensitivity 100%, specificity 95%, positive predictive value 90.5%, and negative predictive value 100%. The IgG detection with Enzygnost was: sensitivity 98%, specificity 100%, positive predictive value 100%, and negative predictive value 91.7%. Only two subjects had positive IgA. The Enzygnost test is an efficient method for the diagnosis of EBV infection although a few IgM false positives can occur. J. Clin. Lab. Anal. 13:65–68, 1999. © 1999 Wiley‐Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|