Tranexamic acid has positive effect in early period of tendon healing by stimulating the tumor necrosis factor-alpha and matrix metalloproteinase-3 expression levels
| Autor: | Havva Erdem, Pınar Naile Gürgör, Orhan Baş, Abdullah Alper Şahin, Erdal Uzun, Alper Çıraklı |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Matrix Metalloproteinase 3
Male medicine.medical_specialty Period (gene) Administration Topical Surgical Wound Urology Achilles Tendon tranexamic acid Transforming Growth Factor beta medicine tendon healing Animals Orthopedics and Sports Medicine Rats Wistar Retrospective Studies Wound Healing business.industry Tumor Necrosis Factor-alpha Rehabilitation Tendon Staining Rats Matrix metalloproteinase-3 medicine.anatomical_structure Tenotomy Treatment Outcome Orthopedic surgery transforming growth factor-beta Immunohistochemistry Surgery Tumor necrosis factor alpha Original Article business Tranexamic acid medicine.drug |
| Zdroj: | Joint Diseases and Related Surgery |
| ISSN: | 2687-4792 2687-4784 |
| Popis: | OBJECTIVES This study aims to evaluate the effect of tranexamic acid (TXA) application in tendon healing by using its immunohistochemical effects on tumor necrosis factor-alpha (TNF-α), matrix metalloproteinase-3 (MMP-3), and transforming growth factor-beta (TGF-β) expression; and to identify if TNF-α, MMP-3, and TGF-β can be used to monitor and evaluate tendon healing or not in tenotomized rat Achilles tendons. MATERIALS AND METHODS Twelve male Wistar-Albino rats (age 6-7-month-old; weighing 300-350 g) were used in this retrospective study conducted between November 2016 and May 2017. The rats were divided into two groups with similar weights. The right legs of the rats were determined as the study group (TXA), and the left legs as the control serum physiologic (SP) group. Under anesthesia, bilateral Achilles tenotomy was performed and surgically repaired. 1 mL of TXA was applied locally for the right side and 1 mL of SP was locally applied for the left side. Half of the rats were sacrificed at the third week (right leg-TXA3, left leg-SP3) and the other half at sixth week (right leg-TXA6, left leg-SP6) and tendon samples were taken from the extremities. Immunohistochemical findings of TNF-α, MMP-3, and TGF-β were evaluated on the basis of the frequency and intensity of staining. RESULTS In TNF-α and MMP-3 and TXA groups, there was a significant difference in staining compared to SP groups (p |
| Databáze: | OpenAIRE |
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