Sequence analysis and overexpression of a pectin lyase gene (pel1) from Aspergillus oryzae KBN616
| Autor: | Kunio Ohmiya, Norihiro Tsukagoshi, Shoko Yoshino-Yasuda, Noriyuki Kitamoto |
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| Rok vydání: | 2001 |
| Předmět: |
Sequence analysis
Aspergillus oryzae Genes Fungal Molecular Sequence Data Gene Expression Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Amino Acid Sequence Molecular Biology Peptide sequence Pectin lyase Polysaccharide-Lyases chemistry.chemical_classification biology Base Sequence Organic Chemistry Structural gene Aspergillus niger Nucleic acid sequence food and beverages General Medicine Sequence Analysis DNA biology.organism_classification Molecular biology Amino acid chemistry Electrophoresis Polyacrylamide Gel Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 65(1) |
| ISSN: | 0916-8451 |
| Popis: | A gene (pel1) encoding pectin lyase (Pel1) was isolated from a shoyu koji mold, Aspergillus oryzae KBN616, and characterized. The structural gene comprised 1,196 bp with a single intron. The ORF encoded 381 amino acids with a signal peptide of 20 amino acids. The deduced amino acid sequence showed high similarity to those of Aspergillus niger pectin lyases and Glomerella cingulata PnlA. The pel1 gene was successfully overexpressed under the promoter of the A. oryzae TEF1 gene. The molecular mass of the recombinant pectin lyase substantially coincided with that calculated based on nucleotide sequence. |
| Databáze: | OpenAIRE |
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