Assessment of thymic output in common variable immunodeficiency patients by evaluation of T cell receptor excision circles
| Autor: | Vanessa Guazzi, Marina Pierdominici, Ivano Mezzaroma, Fernando Aiuti, R. Fantini, Francesca Mazzetta, Grazia Andolfi, Alessandro Aiuti, Alessandra Mortellaro, Marco Marziali |
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| Přispěvatelé: | Guazzi, V, Aiuti, F, Mezzaroma, I, Mazzetta, F, Andolfi, G, Mortellaro, A, Pierdominici, M, Fantini, R, Marziali, M, Aiuti, Alessandro |
| Rok vydání: | 2002 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Naive T cell Immunology Receptors Antigen T-Cell diseases gene immunodeficiency lymphocytes rearrangement t t lymphocytes thymus Thymus Gland CD8-Positive T-Lymphocytes Biology Gene Rearrangement T-Lymphocyte Antigen T-Lymphocyte Subsets Clinical Studies medicine Humans Immunology and Allergy L-Selectin Immunodeficiency T-cell receptor excision circles Common variable immunodeficiency hemic and immune systems T lymphocyte Gene rearrangement Middle Aged medicine.disease Common Variable Immunodeficiency Case-Control Studies Leukocyte Common Antigens Female Biomarkers CD8 |
| Zdroj: | Clinical and Experimental Immunology. 129:346-353 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.2002.01893.x |
| Popis: | SUMMARYCommon variable immunodeficiency (CVID) is a heterogeneous syndrome characterized by repeated infections and hypogammaglobulinaemia. Additionally, T-cell abnormalities including lymphopenia, decreased proliferation to mitogens and antigens, and the reduced production and expression of cytokines, have also been observed. In this study we have investigated the expression of naive, memory and activation markers in T-cell subpopulations in 17 CVID patients in comparison to age-matched normal controls. The numbers of CD4+ T cells, including CD45RA+CD62L+ and, to a lesser extent, CD45RA–CD62L+/RA+CD62L– were significantly reduced in patients, whereas CD8+ T cells were within normal range. In contrast, HLA-DR+ cells were increased both in CD4+ and CD8+ T cells. To assess the thymic output, we analysed the presence of T-cell receptor excision circles (TRECs) in CD4+ and CD8+ T cells by quantitative PCR. TRECs were decreased significantly in patients and the rate of TREC loss was higher with increasing age. TRECs correlated with naive CD4+ T cells, whereas there was an inverse relationship between TRECs and CD8+HLA–DR+ and CD8+CD45RA–CD62L+/RA+CD62L– T cells. Our results suggest the presence of a defect in the naive T cell compartment with origin at the thymic level in CVID, and indicate that TREC may be a useful marker to monitor thymic function in this primary immunodeficiency. |
| Databáze: | OpenAIRE |
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