Two Advanced Cryogenic Procedures for Improving Stevia rebaudiana (Bertoni) Cryopreservation
| Autor: | Raffaella Petruccelli, Lara S. O. Carvalho, Soumaya El merzougui, Carla Benelli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine long-term conservation Ecology Chemistry Botany apical shoot tips axillary shoot tips Plant Science 01 natural sciences Cryopreservation 03 medical and health sciences Horticulture Stevia rebaudiana 030104 developmental biology Genetic resources QK1-989 Shoot droplet-vitrification PVS2 vitrification Vitrification Ecology Evolution Behavior and Systematics V cryo-plate 010606 plant biology & botany Explant culture |
| Zdroj: | Plants Volume 10 Issue 2 Plants, Vol 10, Iss 277, p 277 (2021) Plants 10 (2021): Article 277. doi:10.3390/plants10020277 info:cnr-pdr/source/autori:Benelli C., Carvalho L.S.O., Merzougui S.E.L., Petruccelli R./titolo:Two advanced cryogenic procedures for improving stevia rebaudiana (Bertoni) cryopreservation/doi:10.3390%2Fplants10020277/rivista:Plants/anno:2021/pagina_da:Article 277/pagina_a:/intervallo_pagine:Article 277/volume:10 |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants10020277 |
| Popis: | Cryopreservation is a useful tool for the long-term storage of plant genetic resources, and different cryogenic procedures have recently been developed. The present study focused on the use of the Droplet-vitrification (DV) and V cryo-plate protocol for the cryopreservation of Stevia rebaudiana in vitro-derived apical shoot tips and axillary shoot tips. A preliminary test showed that 90 and 120 min PVS2 (Plant Vitrification Solution 2) treatment significantly reduced the regrowth of the explants before immersion in liquid nitrogen (LN). For both procedures tested, the best osmoprotective condition for obtaining a higher regrowth of cryopreserved explants occurred when explants were PVS2 treated for 60 min. After direct immersion in LN, thawing and plating, the highest regrowth recorded was 80% with DV and 93% with V cryo-plate. Moreover, shoot tips proved to be a more suitable material for Stevia cryopreservation. A satisfactory vegetative regrowth was observed in the subcultures following cryopreservation by DV and V cryo-plate cryogenic procedures. |
| Databáze: | OpenAIRE |
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