Influence of glucose and inhibitors of glycolysis on release of total proteins and enzymes from human leukocytes
| Autor: | Mohamed Lahrichi, Gérard Siest, Y. Houpert, Pierrette Tarallo |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Adult
Blood Glucose Cytoplasm Bicarbonate Clinical Biochemistry Cell Iodoacetates Biology In Vitro Techniques Cytoplasmic Granules Biochemistry Enzyme activator chemistry.chemical_compound DHAP medicine Leukocytes Humans Glycolysis chemistry.chemical_classification Biochemistry (medical) General Medicine Blood Proteins Enzymes Metabolic pathway Enzyme medicine.anatomical_structure Glucose chemistry Anaerobic glycolysis Depression Chemical Sodium Fluoride |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 79(2) |
| ISSN: | 0009-8981 |
| Popis: | We studied the influence of substrates and glycolysis inhibitors on the release of proteins and enzymes from human leukocytes. We show the relationship between metabolic level and percentage of enzyme release. Leukocyte kept alive in a nutrient-free bicarbonate medium liberate enzymes even though their metabolic state is satisfactory. The addition of glucose to the medium significantly decreases this phenomenon without affecting the energy level. Addition of glycolysis inhibitors increases it, reduces the energy level, and interferes with other metabolic pathways. The results are discussed and compared to those obtained by other authors using various cell models. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|