Measles virus transmembrane fusion protein synthesized de novo or presented in immunostimulating complexes is endogenously processed for HLA class I- and class II-restricted cytotoxic T cell recognition
| Autor: | Fons G. C. M. Uytdehaag, R.S. van Binnendijk, Jolande Boes, C. A. van Baalen, A.D.M.E. Osterhaus, P de Vries, Martien C. M. Poelen, Vincenzo Cerundolo |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
biology
Paramyxoviridae CD8 Antigens Histocompatibility Antigens Class I Immunology Antigen presentation Histocompatibility Antigens Class II Antigen-Presenting Cells Articles Lymphocyte Activation biology.organism_classification Virology Virus Measles virus Antigen CD4 Antigens Animals Humans Immunology and Allergy Cytotoxic T cell Antigen-presenting cell Viral Fusion Proteins CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Scopus-Elsevier The Journal of Experimental Medicine |
| DOI: | 10.1084/jem.176.1.119 |
| Popis: | The routes used by antigen-presenting cells (APC) to convert the transmembrane fusion glycoprotein (F) of measles virus (MV) to HLA class I and class II presentable peptides have been examined, using cloned cytotoxic T lymphocytes in functional assays. Presentation by Epstein-Barr virus-transformed B lymphoblastoid cell lines was achieved using live virus, ultraviolet light-inactivated virus, and purified MV-F delivered either as such or incorporated in immunostimulating complexes (MV-F-ISCOM). Only live virus and MV-F-ISCOM allow presentation by class I molecules, while all antigen preparations permit class II-restricted presentation. We observe presentation of MV-F from live virus and as MV-F-ISCOM by class II molecules in a fashion that is not perturbed by chloroquine. Our studies visualize novel presentation pathways of type I transmembrane proteins. |
| Databáze: | OpenAIRE |
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