The Spectrum-Effect integrated fingerprint of Polygonum cuspidatum based on HPLC-diode array detection-flow injection-chemiluminescence
| Autor: | Xiao-Ping Ding, Qi Jin, Min-Jian Qin, Li-Qiong Sun, Cui-Ling Zhang, Bo-Yang Yu |
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| Rok vydání: | 2013 |
| Předmět: |
Quality Control
Flow injection analysis Luminescence Chromatography Chemistry Objective method General Medicine High-performance liquid chromatography Diode array Antioxidants law.invention Hplc esi ms Complementary and alternative medicine law Fingerprint Fallopia japonica Flow Injection Analysis Drug Discovery Chromatography High Pressure Liquid POLYGONUM CUSPIDATUM Drugs Chinese Herbal Chemiluminescence |
| Zdroj: | Chinese Journal of Natural Medicines. 11:546-552 |
| ISSN: | 1875-5364 |
| Popis: | Aim To establish the Spectrum-Effect integrated fingerprint of Polygonum cuspidatum to evaluate the quality of P. cuspidatum . Methods An on-line HPLC–DAD–flow injection chemiluminescence (FICL) method was developed to investigate the quality of P. cuspidatum from different habitats based on the established Spectrum-Effect integrated fingerprint. Results Nineteen batches of samples of P. cuspidatum were evaluated for the similarity of their chromatographic and free radical scavenging fingerprints, and the results compared. Main antioxidants were estimated by regression analysis between peak areas of thirteen compounds and their activities. Some active compounds were identified by HPLC-ESI-MS. Consulsions The results indicated that main antioxidants in P. cuspidatum could be rapidly screened by the established Spectrum-Effect integrated fingerprint based on on-line HPLC-DAD-FICL, and would be more efficient and objective method to evaluate the quality of P. cuspidatum . |
| Databáze: | OpenAIRE |
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