The bond in the bacteriophage øX174 gene A protein-DNA complex is a tyrosyl-5'-phosphate ester
| Autor: | H.S. Jansz, G. H. Veeneman, J. H. Van Boom, H.A.A.M. van Teeffelen, P.D. Baas, A.D.M. van Mansfeld |
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| Jazyk: | angličtina |
| Předmět: |
Genes
Viral viruses Biophysics Bacteriophage phi X174 Plasma protein binding Biology Biochemistry DNA sequencing Bacteriophage Viral Proteins chemistry.chemical_compound Structural Biology Genetics Nucleotide Molecular Biology Gene chemistry.chemical_classification Base Sequence Cell Biology biology.organism_classification chemistry Covalent bond DNA Viral Tyrosine Bacteriophage phi X 174 DNA Protein Binding |
| Zdroj: | FEBS Letters. (2):351-356 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(84)80804-2 |
| Popis: | The bacteriophage øX174 gene A protein cleaves the viral strand of the double-stranded replicative form (RF) DNA of the phage at a specific site, the origin. It leaves a free 3'-OH at nucleotide 4305 (G) of the øx DNA sequence and binds covalently to the DNA. The nature and position of the covalent bond have been determined using the octadecadesoxyribonucleotide CAACTTG[32P]ATATTAATAAC. This octadecamer, which corresponds to nucleotides 4299–4316 of øX viral DNA, is cleaved by gene A protein. Gene A protein is bound to the labelled phosphate via a tyrosyl residue, indicating that binding occurs to the nucleotide corresponding to 4306 (A) of the øX viral DNA strand.Bacteriophage øX174Gene A proteinDNA replicationProtein-DNA complexSynthetic oligonucleotideTyrosyl-5'-phosphate ester |
| Databáze: | OpenAIRE |
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