Primary structure of Neurospora crassa gamma-tubulin
| Autor: | Eckhard Kube-Granderath, Sabine Heckmann, Manfred Schliwa |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA
Complementary Genes Fungal Molecular Sequence Data Gene Dosage macromolecular substances Neurospora crassa Tubulin Genetics Northern blot Amino Acid Sequence RNA Messenger Cloning Molecular Phylogeny Southern blot biology Phylogenetic tree Base Sequence fungi Protein primary structure Crassa Nucleic acid sequence RNA Fungal General Medicine Sequence Analysis DNA biology.organism_classification Molecular biology Biochemistry biology.protein |
| Zdroj: | Gene. 199(1-2) |
| ISSN: | 0378-1119 |
| Popis: | We report the isolation and characterization of a cDNA-clone for γ -tubulin from N. crassa . The deduced amino-acid sequence of the cDNA-clone most resembles that of γ -tubulins from other fungi. Differential Southern blot analysis demonstrates that most probably a single gene represents γ -tubulin. In addition, Northern blot analysis shows that most probably only one single mRNA encoding γ -tubulin is transcribed in N. crassa . In a phylogenetic tree that includes all known γ -tubulins, N. crassa γ -tubulin is grouped, as expected, together with other fungal γ -tubulins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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