Acute Downregulation of Novel Hypothalamic Protein Sushi Repeat-Containing Protein X-Linked 2 after Experimental Traumatic Brain Injury
| Autor: | Asla Pitkänen, Leonardo Lara-Valderrábano, Jenni Karttunen, Xavier Ekolle Ndode-Ekane, Mehwish Anwer, Noora Puhakka |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Traumatic brain injury business.industry Protein x Hypothalamus Down-Regulation Membrane Proteins medicine.disease nervous system diseases Neoplasm Proteins Rats Rats Sprague-Dawley Endocrinology nervous system Downregulation and upregulation Internal medicine Brain Injuries Traumatic medicine Endocrine system Animals Neurology (clinical) business Biomarkers |
| Zdroj: | Journal of neurotrauma. 37(7) |
| ISSN: | 1557-9042 |
| Popis: | Traumatic brain injury (TBI) causes damage to the hypothalamo-hypophyseal axis, leading to endocrine dysregulation in up to 40% of TBI patients. Hence, there is an urgent need to identify non-invasive biomarkers for TBI-associated hypothalamo-hypophyseal pathology. Sushi repeat-containing protein X-linked 2 (SRPX2) is a novel hypothalamic protein expressed in both rat and human brain. Our objective was to investigate the effect of acquired brain injury on plasma SRPX2 protein levels and SRPX2 expression in the brain. We induced severe lateral fluid-percussion injury in adult male rats and investigated changes in SRPX2 expression at 2 h, 6 h, 24 h, 48 h, 72 h, 5 days, 7 days, 14 days, 1 month, and 3 months post-injury. The plasma SRPX2 level was assessed by Western blot analysis. Hypothalamic SRPX2-immunoreactive neuronal numbers were estimated from immunostained preparations. At 2 h post-TBI, plasma SRPX2 levels were markedly decreased compared with the naïve group (area under the curve = 1.00 |
| Databáze: | OpenAIRE |
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