Examining the Effect of Heat Stress on Montastraea cavernosa (Linnaeus 1767) from a Mesophotic Coral Ecosystem (MCE)
| Autor: | Sango Otieno, Sok Kean Khoo, Kevin B. Strychar, John E. Skutnik |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
lcsh:Hydraulic engineering stress-genes Coral bleaching Coral Geography Planning and Development Aquatic Science global warming 01 natural sciences Biochemistry Microbiology 03 medical and health sciences lcsh:Water supply for domestic and industrial purposes lcsh:TC1-978 Heat shock protein Gene expression 030304 developmental biology Water Science and Technology Montastraea cavernosa 0303 health sciences lcsh:TD201-500 biology 010604 marine biology & hydrobiology coral bleaching qRT-PCR biology.organism_classification mesophotic coral ecosystems (MCEs) Hsp90 immunity Real-time polymerase chain reaction Catalase biology.protein |
| Zdroj: | Water, Vol 12, Iss 1303, p 1303 (2020) Water Volume 12 Issue 5 |
| ISSN: | 2073-4441 |
| Popis: | Coral reefs are under increasing pressure from global warming. Little knowledge, however, exists regarding heat induced stress on deeper mesophotic coral ecosystems (MCEs). Here, we examined the effect of acute (72 h) and chronic (480 h) heat stress on the host coral Montastraea cavernosa (Linnaeus 1767) collected from an upper MCE (~30 m) in Florida, USA. We examined six immune/stress-related genes: ribosomal protein L9 (RpL9), ribosomal protein S7 (RpS7), B-cell lymphoma 2 apoptosis regulator (BCL-2), heat shock protein 90 (HSP90), catalase, and cathepsin L1, as a proxy for coral response to heat stress. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate the gene expression. Overall, both acute and chronic heat stress treatments elicited a response in gene expression relative to control samples. Acute heat exposure resulted in up-regulation of catalase, BCL-2, and HSP90 at all time points from hour 24 to 48, suggesting the activation of an oxidative protective enzyme, molecular chaperone, and anti-apoptotic protein. Fewer genes were up-regulated in the chronic experiment until hour 288 (30 ° C) where catalase, RpL9, and RpS7 were significantly up-regulated. Chronic heat exposure elicited a physiological response at 30 ° C, which we propose as a heat-stress threshold for Montastraea cavernosa (M. cavernosa) collected from an MCE. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|