Preparation and characterization of gamma oryzanol loaded zein nanoparticles and its improved stability
| Autor: | Vilai Rungsardthong, Dudsadee Uttapap, Ubonphan Rodsuwan, Usaraphan Pithanthanakul, Korawinvich Boonpisuttinant, Benjawan Thumthanaruk, Savitri Vatanyoopaisarn, Krittiya Thisayakorn |
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| Rok vydání: | 2020 |
| Předmět: |
Scanning electron microscope
Nanoparticle lcsh:TX341-641 02 engineering and technology zein protein gamma oryzanol zeta potential Ingredient 0404 agricultural biotechnology Zeta potential Original Research Chemistry Rice bran oil food and beverages 04 agricultural and veterinary sciences dispersion nanoparticles nano-encapsulation 021001 nanoscience & nanotechnology 040401 food science Chemical engineering Transmission electron microscopy Yield (chemistry) Particle size nano‐encapsulation 0210 nano-technology lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Food Science & Nutrition, Vol 9, Iss 2, Pp 616-624 (2021) Food Science & Nutrition, 9(2):616-624 Food Science & Nutrition |
| ISSN: | 2048-7177 |
| Popis: | Gamma oryzanol (GO), a bioactive ingredient found in rice bran oil, performs a variety of biological effects such as antioxidant activity, reduction of total cholesterol, anti‐inflammation, and antidiabetes. However, GO is water‐insoluble and normally degrades through oxidation. Thus a nano‐encapsulation technique was investigated to improve its stability and quality. In this research, gamma oryzanol was successfully encapsulated into zein nanoparticles. The fabrication parameters including pH, zein concentration (0.3, 0.4, and 0.5% w/v), and % GO loading (30, 40, and 50% by weight) were investigated. Particle size, zeta potential, yield, encapsulation efficiency and the stability or GO retention during the storage were determined. The morphology of gamma oryzanol loaded zein nanoparticles (GOZNs) was observed by scanning electron micrographs and transmission electron microscope. The increase of zein concentration and % GO loading resulted to an increase of yield, encapsulation efficiency, and particle size. The particle size of the GOZNs ranged from 93.24–350.93, and 144.13–833.27, and 145.27–993.13 nm for each zein concentration with 3 loading levels, respectively. Nano‐encapsulation exhibited higher % GO retention compared with nonencapsulated GO during 60 days storage both at 4°C and −18°C. In vitro study indicated the sustained release of GO in the simulated gastric fluid followed by simulated intestinal fluid. This finding indicated a high potential for the application of insoluble GO with improved stability by encapsulation with the hydrophobic zein protein. The bioactive compound and gamma oryzanol (GO) were encapsulated in zein nanoparticles (ZNs) to obtain gamma oryzanol loaded zein nanoparticles (GOZNs), using a liquid‐liquid dispersion. The effect of pH, zein concentration, and % GO loading on the properties of GOZNs showed that the increase of zein concentrations and % GO loading resulted in larger sizes of the nanoparticles as well as the increased % encapsulation efficiency and % yield of GOZNs. The encapsulation results in the higher stability of GO during 60 days storage. |
| Databáze: | OpenAIRE |
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