Stage-Specific miRs in Chondrocyte Maturation: Differentiation-Dependent and Hypertrophy-Related miR Clusters and the miR-181 Family
| Autor: | Martin Ruetze, Jessica Gabler, Solvig Diederichs, Wiltrud Richter, Katharina Lydia Kynast, Tobias Grossner |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Cellular differentiation Biomedical Engineering Bioengineering Biochemistry Chondrocyte Biomaterials Chondrocytes Transforming Growth Factor beta Osteoarthritis microRNA medicine Humans Aged Aged 80 and over Regulation of gene expression biology Mesenchymal stem cell Cell Differentiation Hypertrophy Transforming growth factor beta Middle Aged Chondrogenesis Embryonic stem cell Cell biology MicroRNAs medicine.anatomical_structure Gene Expression Regulation Immunology biology.protein Female Collagen Type X |
| Zdroj: | Tissue Engineering Part A. 21:2840-2851 |
| ISSN: | 1937-335X 1937-3341 |
| Popis: | Human mesenchymal stromal cells (hMSC) differentiating toward the chondrogenic lineage recapitulate successive phases of embryonic chondrocyte maturation developing from progenitor cells to hypertrophic chondrocytes. Osteoarthritic cartilage is characterized by an alteration in chondrocyte metabolism and upregulation of hypertrophic differentiation markers. A number of studies point toward a functional role for microRNAs (miRs) in controlling chondrocyte differentiation and development of osteoarthritis (OA). However, information on miRs that may regulate a specific phase of chondrocyte maturation, especially hypertrophy, is lacking. We here aimed to unravel miR profiles modulated during chondrogenesis of hMSC to obtain new differentiation markers and potential new targets relevant for differentiation outcome and OA development. hMSC were subjected to transforming growth factor-β (TGF-β)-driven chondrogenesis and miR profiles were determined by microarray analysis at distinct developmental time points. Expression of selected miRs was compared to cultures lacking chondrogenesis and to redifferentiated nonhypertrophic articular chondrocytes. Among 1349 probed miRs, 553 were expressed and 169 (31%) were significantly regulated during chondrogenesis. Hierarchical clustering identified specific miR expression patterns representative for MSC, prechondrocytes, chondroblasts, chondrocytes, and hypertrophic chondrocytes, respectively. Regulation of miR-181 family members allowed discrimination of successive differentiation stages. Levels of several miRs, including miR-23b, miR-140, miR-181, and miR-210 positively correlated with successful chondrocyte formation. Hypertrophic MSC-derived chondrocytes and nonhypertrophic articular chondrocytes showed differential expression of miR-181a, miR-210, and miR-31, but not miR-148a implicated in COL10A1-regulation. We conclude that the here identified stage-dependent miR clusters may have imperative functions during chondrocyte differentiation providing novel diagnostic tools and targets of potential relevance for OA development. |
| Databáze: | OpenAIRE |
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