Extraction, identification, and antioxidant property evaluation of limonin from pummelo seeds
| Autor: | Minyi Tang, Xi Sun, Si Qin, Fangming Deng, Qingshan Wang, Zhibing Zheng, Chenghao Lv |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Identification Antioxidant Limonin Nrf2-ARE pathway medicine.medical_treatment Extraction The journal is particularly indebted to Prof. De-Xing Hou of Kagoshima University Japan and Prof. Layi Adeola of Purdue University USA for their skills and dedication in organizing and editing the papers 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Food Animals Acetone medicine Response surface methodology lcsh:SF1-1100 Chromatography 010401 analytical chemistry Extraction (chemistry) 0104 chemical sciences 030104 developmental biology chemistry Phytochemical Yield (chemistry) Hepg2 cells Animal Science and Zoology lcsh:Animal culture |
| Zdroj: | Animal Nutrition, Vol 4, Iss 3, Pp 281-287 (2018) Animal Nutrition |
| ISSN: | 2405-6545 |
| Popis: | Limonin, the main bioactive phytochemical constituent of limonoids with multi-functions, is enriched in citrus fruits and often found at a high concentration in citrus seeds. The present study was attempted to introduce a new and efficient extraction method to isolate limonoids from pummelo seeds, and to evaluate the antioxidant property of the main constituent limonin in HepG2 cells. Three key single factors were identified for the extraction of limonoids from pummelo seeds using the Box-Behnken experiment design of response surface methodology (RSM), and the optimized extraction parameters were treatment with 89.68 mL of anhydrous acetone for 4.62 h at 78.94 °C, while the yield of limonoids was 11.52 mg/g. The structure of isolated main constituent of the limonoids was further identified as limonin by Fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance (NMR) spectrum. Moreover, the molecular data in HepG2 cells revealed that limonin exerted its anti-oxidant property mainly by the activation of nuclear factor (erythroid-2)-like 2 (Nrf2)/kelch-like ECH-associated protein 1 (Keap1)- antioxidant response element (ARE) pathway in the form of transcriptional regulation of Nrf2 mRNA and posttranscriptional regulation of Nrf2/Keap1 system. These results demonstrate that pummelo seeds are an ideal source of limonoids, and limonin is proved to exert its anti-oxidant property by the activation of Nrf2/Keap1 pathway. Keywords: Limonin, Extraction, Identification, Nrf2-ARE pathway, Antioxidant |
| Databáze: | OpenAIRE |
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