Acyl‐CoA synthetase 6 regulates long‐chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic processes in mice
| Autor: | Shinnosuke Matsueda, Takeshi Matsui, Azusa Inoue, Makoto Arita, Kyosuke Shishikura, Sayoko Kuroha, Hachiro Iseki |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine endocrine system Docosahexaenoic Acids Apoptosis Fertilization in Vitro testis Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine ACSL6 Coenzyme A Ligases Genetics Animals docosapentaenoic acid Spermatogenesis Molecular Biology Phospholipids Mice Knockout chemistry.chemical_classification Sperm Count urogenital system Research Cell Membrane Metabolism docosahexaenoic acid Spermatids Sperm Cell biology 030104 developmental biology Enzyme Gene Expression Regulation chemistry Docosahexaenoic acid Fatty Acids Unsaturated Oocytes lipids (amino acids peptides and proteins) Docosapentaenoic acid 030217 neurology & neurosurgery Biotechnology Polyunsaturated fatty acid |
| Zdroj: | The FASEB Journal |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | Long-chain polyunsaturated fatty acids (LCPUFAs), such as docosahexaenoic acid (DHA, 22:6) and docosapentaenoic acid (DPA, 22:5), have versatile physiologic functions. Studies have suggested that DHA and DPA are beneficial for maintaining sperm quality. However, their mechanisms of action are still unclear because of the poor understanding of DHA/DPA metabolism in the testis. DHA and DPA are mainly stored as LCPUFA-containing phospholipids and support normal spermatogenesis. Long-chain acyl-conenzyme A (CoA) synthetase (ACSL) 6 is an enzyme that preferentially converts LCPUFA into LCPUFA-CoA. Here, we report that ACSL6 knockout (KO) mice display severe male infertility due to attenuated sperm numbers and function. ACSL6 is highly expressed in differentiating spermatids, and ACSL6 KO mice have reduced LCPUFA-containing phospholipids in their spermatids. Delayed sperm release and apoptosis of differentiated spermatids were observed in these mice. The results of this study indicate that ACSL6 contributes to the local accumulation of DHA- and DPA-containing phospholipids in spermatids to support normal spermatogenesis.-Shishikura, K., Kuroha, S., Matsueda, S., Iseki, H., Matsui, T., Inoue, A., Arita, M. Acyl-CoA synthetase 6 regulates long-chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic processes in mice. |
| Databáze: | OpenAIRE |
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