Analytical Study of the Antifungal Posaconazole in Raw Material: Quantitative Bioassay, Decomposition Chemical Kinetics, and Degradation Impurities by LC-QTOF-MS
| Autor: | Cássia Virginia Garcia, Sendy Sales Oliveira, Andreas Sebastian Loureiro Mendez, Andressa da Silva Bitencourt, Caren Gobetti |
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| Rok vydání: | 2020 |
| Předmět: |
Posaconazole
Antifungal Agents Kinetics Triazole Antifungal drug Mass spectrometry 01 natural sciences Mass Spectrometry Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound Drug Stability medicine Environmental Chemistry Bioassay Chromatography High Pressure Liquid Pharmacology Chromatography 010405 organic chemistry Chemistry 010401 analytical chemistry Triazoles 0104 chemical sciences Mass spectrum Biological Assay Agronomy and Crop Science Food Science medicine.drug Chromatography Liquid |
| Zdroj: | Journal of AOAC International. 104(4) |
| ISSN: | 1944-7922 |
| Popis: | Background Posaconazole is a triazole antifungal drug that was approved by the U.S. Food and Drug Administration in 2006. No bioassay of it is available in the literature nor official codes for potency determination in bulk. Objective To conduct an analytical study focused on posaconazole in bulk. Methods An alternative microbiological assay was validated for drug quantitation, applying agar diffusion technics (3 × 3 design), using Saccharomyces cerevisiae ATCC MYA 1942 as a test microorganism (2% inoculum). An isocratic HPLC–DAD method, with C8 Shim-pack column (250 × 4.6 mm, 5 μm) and methanol–water (75:25 v/v) mobile phase was used for stress stability by photolysis and oxidation, indicating the formation of degradation products, which were investigated by ultra-performance liquid chromatography to quadrupole time-of-flight mass spectrometry. Results The established conditions for the bioassay were satisfactory. It was linear in the range evaluated (2.5–10.0 µg/mL), as well as precise, accurate, and robust. Stress tests showed drug susceptibility to the factors evaluated (60% of degradation after 120 min). Kinetics curves for photolytic decomposition followed first-order kinetics. From a photolytic and oxidative degraded matrix, three major degradation products were identified as being derivatives with modifications in the piperazine central ring and in the triazole and triazolone side chains, whose mass spectra results were m/z 683 (DP1), m/z 411 (DP2), and m/z 465 (DP3). Conclusions The microbiological method was adequately validated and demonstrated to be equivalent to physico-chemical ones. The impurities found are described for the first time in studies with posaconazole raw material. Highlights A microbiological bioassay was developed for posaconazole, first-order kinetics was determined for photolytic degradation, and structures for new degradation products were suggested. |
| Databáze: | OpenAIRE |
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