DNA barcoding evaluation and implications for phylogenetic relationships in ladybird beetles (Coleoptera: Coccinellidae)
| Autor: | Xiao-Ping Yu, Hang-Feng Zhu, Zi-Ye Wang, Zheng-Liang Wang, Tian-Zhao Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Paraphyly Chilocorinae Phylogenetic tree Cytochrome c oxidase subunit I Biology biology.organism_classification 010603 evolutionary biology 01 natural sciences DNA barcoding Coleoptera Electron Transport Complex IV 03 medical and health sciences Monophyly 030104 developmental biology Phylogenetics Evolutionary biology Genetics Coccinellidae Animals DNA Barcoding Taxonomic Insect Proteins Molecular Biology Phylogeny |
| Zdroj: | Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis. 30(1) |
| ISSN: | 2470-1408 |
| Popis: | Ladybird beetles (Coleoptera: Coccinellidae), with broad morphological diversity, wide geographic distribution and substantial agricultural significance, are a challenging group for taxonomists and phylogenetics. As a promising tool to identify and discover new species, DNA barcoding might offer significant potential for identification, taxonomy and phylogeny of ladybird beetles. In the present study, a total of 1364 COI (cytochrome C oxidase subunit I) sequences representing 128 species from 52 genera of ladybird beetles were screened for barcoding evaluation and phylogenetic analysis. Our results from the barcoding analysis revealed that COI displays a similar level of species identification efficiency (nearly 90%) either based on Kimura two-parameter (K2P) distances calculation or on simplified neighbour-joining (NJ) tree construction. The phylogenetic relationships within the family Coccinellidae was analyzed by Bayesian-inference (BI) method. The phylogenetic results confirmed the monophyly of the subfamilies Microweisinae and Coccinellinae sensu Ślipinski (2007), and suggested that the subfamilies Coccidulinae, Chilocorinae and Scymninae are paraphyletic. However, the phylogenetic relationships among different subfamilies are not clearly defined and thus remain to be thoroughly studied. Overall, our study confirmed the usefulness of DNA barcoding for coccinellid species identification and phylogenetic inference. |
| Databáze: | OpenAIRE |
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