Gene expression changes during step-wise differentiation of embryonic stem cells along the inner ear hair cell pathway

Autor:	Gordon K. Smyth, Michelle R Newman, Matthew E. Ritchie, Michelle G. de Silva, Michael S. Hildebrand, Helen Christopoulos, Katrina M. Bell, Hans-Henrik M. Dahl
Rok vydání:	2006
Předmět:	Pluripotent Stem Cells Cellular differentiation Glucose Transport Proteins Facilitative Neuroectodermal Cell Biology Cell Line Mice otorhinolaryngologic diseases medicine Animals Osteonectin Induced pluripotent stem cell HSP47 Heat-Shock Proteins Organ of Corti Embryonic Stem Cells Cochlea Oligonucleotide Array Sequence Analysis Genetics Hair Cells Auditory Inner Epidermal Growth Factor Tissue Engineering Cell Differentiation General Medicine Embryonic stem cell Up-Regulation Cell biology medicine.anatomical_structure Gene Expression Regulation Otorhinolaryngology sense organs Hair cell Stem cell Cell Division
Zdroj:	Acta Oto-Laryngologica. 126:1148-1157
ISSN:	1651-2251 0001-6489
DOI:	10.1080/00016480600702118
Popis:	Our study outlines an alternative approach for the selection and investigation of genes involved in inner ear function.To gain understanding of the gene pathways involved in the development of the normal cochlea.Microarray technology currently offers the most efficient approach to investigate gene expression and identify pathways involved in cell differentiation. Epidermal growth factor (EGF) induces cultures derived from the organ of Corti to proliferate and produce new hair cells. Since pluripotent embryonic stem (ES) cells have the capacity to generate all tissues, we induced murine ES cells to differentiate towards ectodermal and neuroectodermal cell types and from there investigated their commitment towards the hair cell lineage in the presence of EGF. Cells were collected at three points along the differentiation pathway and their expression profiles were determined using the Soares NMIE mouse inner ear cDNA library printed in microarray format.Three genes up-regulated after addition of EGF (serine (or cysteine) proteinase inhibitor, clade H, member 1 (Serpinh1), solute carrier family 2 (facilitated glucose transporter), member 10 (Slc2a10) and secreted acidic cysteine-rich glycoprotein (Sparc)) were selected for further analysis and characterization. Of the three genes, Serpinh1 and Slc2a10 have never been implicated in the hearing process.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0ceb55ec9b5d7a29a231bf0d7196244 https://doi.org/10.1080/00016480600702118 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.