Restoring the biophysical properties of decellularized patches through recellularization
| Autor: | Avihai Spizzichino, Hadar Sarig, Hanumakumar Bogireddi, Tomer Bronshtein, Subbu S. Venkatraman, Jacob Bortman, Udi Sarig, Marcelle Machluf, Boey Yin Chiang Freddy, Gigi Chi Ting Au-Yeung, Limor Baruch |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Scaffold Swine Cell Biomedical Engineering Nanotechnology 02 engineering and technology Regenerative medicine Cell Line Extracellular matrix 03 medical and health sciences Tissue engineering Tensile Strength medicine Animals Humans General Materials Science Cell Proliferation Glycosaminoglycans Decellularization Tissue Engineering Tissue Scaffolds Chemistry Myocardium Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Extracellular Matrix Cell biology 030104 developmental biology medicine.anatomical_structure Cellularization 0210 nano-technology |
| Zdroj: | Biomaterials Science. 5:1183-1194 |
| ISSN: | 2047-4849 2047-4830 |
| DOI: | 10.1039/c7bm00208d |
| Popis: | Various extracellular matrix (ECM) scaffolds, isolated through decellularization, were suggested as ideal biomimetic materials for 'Functional tissue engineering' (FTE). The decellularization process comprises a compromise between damaging and preserving the ultrastructure and composition of ECM-previously shown to affect cell survival, proliferation, migration, organization, differentiation and maturation. Inversely, the effects of cells on the ECM constructs' biophysical properties, under physiological-like conditions, remain still largely unknown. We hypothesized that by re-cellularizing porcine cardiac ECM (pcECM, as a model scaffold) some of the original biophysical properties of the myocardial tissue can be restored, which are related to the scaffold's surface and the bulk modifications consequent to cellularization. We performed a systematic biophysical assessment of pcECM scaffolds seeded with human mesenchymal stem cells (MSCs), a common multipotent cell source in cardiac regenerative medicine. We report a new type of FTE study in which cell interactions with a composite-scaffold were evaluated from the perspective of their contribution to the biophysical properties of the construct surface (FTIR, WETSEM™) and bulk (DSC, TGA, and mechanical testing). The results obtained were compared with acellular pcECM and native ventricular tissue serving as negative and positive controls, respectively. MSC recellularization resulted in an inter-fiber plasticization effect, increased protein density, masking of acylated glycosaminoglycans (GAGs) and active pcECM remodelling which further stabilized the reseeded construct and increased its denaturation resistance. The systematic approach presented herein, therefore, identifies cells as "biological plasticizers" and yields important methodologies, understanding, and data serving both as a reference as well as possible 'design criteria' for future studies in FTE. |
| Databáze: | OpenAIRE |
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